Efficacy and safety of inactivated very virulent infectious bursal disease virus in broiler chickens

Chang, Zhi Ning (2022) Efficacy and safety of inactivated very virulent infectious bursal disease virus in broiler chickens. [Project Paper] (Submitted)

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Abstract

Very virulent infectious bursal disease virus (vvIBDV) has led to significant economic losses in the poultry industry worldwide. The development of vaccines against vvIBDV is prioritized as the failure of current IBD vaccines to provide full protection. In this study, the efficacy and safety of inactivated vvIBDV as a candidate vaccine were evaluated. Sixty day-old commercial broiler chickens were grouped into A, B, and C, indicating non-booster, booster, and control respectively. Groups A and B were inoculated with 107 EID50/0.2mL inactivated vvIBDV subcutaneously on day-old, and Group B with booster dose on day 14. On day 28, the birds were subdivided into Group ACH, BCH and CCH, and challenged with a pathogenic field strain of 105 EID50/1.0mL via eye drops (0.2mL) and orally (0.8mL)) to evaluate the candidate vaccine’s effectiveness. The result showed no clinical manifestations for both control and vaccinated groups. There was an overall increase in body weight and bursa weight for all groups, but only the booster group showed an increase in these two parameters post-challenged. Also, only the booster group showed a rise in the bursa-to-body-weight ratio post-challenged. The bursa of Fabricius for all groups appeared normal grossly and histologically. Nevertheless, post-challenged findings revealed the Control group (CCH) ranged from moderate-severe to severe, indicating a positive vvIBDV infections. For the non-booster group (ACH), the lesions observed moderate-severe to severe, while for the booster group (BCH), the lesions observed ranged mild-moderate to moderate. Bursal lesion scoring of all groups were significantly higher post-challenged (p<0.05), but the booster group remained the least changes. Moreover, the control group was recorded with the highest increase in the antibody titre post-challenged which indicated an infection, meanwhile both vaccinated groups showed less increase, with the least changes in booster group. Booster group also showed the least viral shedding among all groups. Thus, the findings suggested that the candidate vaccine was safe and able to provide better immunization with double doses.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 May 2024 08:42
Last Modified:	30 May 2024 08:42
URI:	http://psaspb.upm.edu.my/id/eprint/1902

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