Efficacy of immersion and oral vaccination and their combination against Aeromonas hydrophila in juvenile hybrid red tilapia (Oreochromis sp.)

Abd Rashit, Siti Aishah (2022) Efficacy of immersion and oral vaccination and their combination against Aeromonas hydrophila in juvenile hybrid red tilapia (Oreochromis sp.). [Project Paper] (Submitted)

Text lp FPV 2022 92.pdf Download (2MB)

Abstract

Aeromonas hydrophila is one of the important bacteria infecting the hybrid red tilapia (Oreochromis sp.). While vaccination is a crucial protection of fish against disease, the route by which the vaccine is administered also plays a dependent role in optimizing its efficacy. Therefore, this study aims to differentiate the effects of oral vaccination, immersion vaccination, and their combination against A. hydrophila on juvenile hybrid red tilapia. A total of 75 juvenile hybrid red tilapia of 15g ± 5g were divided into 5 groups, which were categorized into types of administration routes, namely oral vaccination with immersion booster (group OI), immersion then oral booster (IO group), oral then oral booster (group OO), immersion then immersion boosters (group II), and finally an unvaccinated group as a control group. The first vaccination started at week 0, followed by a booster vaccination at week 1, then a challenge with live A. hydrophila at week 2. Skin and gill samples were collected post-challenge for histology to assess the mucosal immune responses. The thickness of epidermis, number of skin-associated lymphoid tissue (SALT), and inflammatory cells size in gills were assessed. Compared to control group, the epidermis thickness and number of SALT were insignificantly different in all groups. However, size of gills’ inflammatory cells in II group were significantly (p<0.05) increased post-challenge. This shows that immersion-immersion vaccination was most attributable to the mucosal immune response. The comparison between these groups supports the hypothesis that there is a correlation between mucosal immune response and different vaccination routes.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	31 May 2024 08:31
Last Modified:	31 May 2024 08:31
URI:	http://psaspb.upm.edu.my/id/eprint/1919

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