Khalid, Suriani (2022) Efficacy of immersion and oral vaccination and their combination against aeromonas hydrophila in juvenile catfish (Clarias sp.). [Project Paper] (Submitted)
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Abstract
Cultivation of catfish is often threatened by disease due to the imbalance in the interactions between fish, environmental conditions and disease-causing organisms. Aeromonas hydrophila infections, also known as MAS (Motile Aeromonas Septicaemia) or red spot disease, result in significant losses to farmers due to mortality, antibiotic treatments, and lost feeding days. Various routes of vaccination against the organism such as immersion or oral route can be ideal methods of preventing the disease. However, the effectiveness and labour costs of each route are still questionable. The aim of the study was to understand the efficacy of immersion and oral vaccination and their combination against A. hydrophila using formalin-killed bacterin in juvenile catfish (Clarias sp.). This study was conducted in 4 weeks. 50 juvenile catfish were equally distributed into 5 treatment groups. Group 1 serves as a combination of immersion and oral vaccination, Group 2 serves as a combination of oral and immersion vaccination, Group 3 serves as immersion vaccination only, Group 4 serves as oral vaccination only, and Group 5 serves as control group. All groups were challenged at week 4 with 100 μL 1 × 109 CFU/mL of live Aeromonas hydrophila via intraperitoneal injection (i.p.). Following challenge, all fish were observed for clinical signs, mortality and bacterial isolation of dead fish. Three fish from each group were sacrificed for sampling. Skin samples were taken for histopathology. Statistical analysis of the distribution of inflammatory cells across the skin tissue showed that there is a significant difference (p<0.05) of inflammatory cells in the skin when comparing the treatment groups to the control group. In summary, both vaccination types did not result in a significant difference (p>0.05) in the development of the lymphoid tissue, since mucosa-associated lymphoid tissues (MALTs) cannot be assessed in a short period of 1 week vaccination interval.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 31 May 2024 08:32 |
| Last Modified: | 31 May 2024 08:32 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1920 |
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