Palani, Khaiyal Vili (2022) Microbiological and its antibiotic susceptibility profiles of buffaloes infected with mastitis in a commercialised dairy buffalo farm. [Project Paper] (Submitted)
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Abstract
The diversity of bacterial composition in buffalo milk in the presence of intramammary infection is not vastly explored in Malaysia as compared with other dairy industry animals such as cattle, goats, and sheep. Moreover, identification of bacterial pathogens associated with bubaline mastitis is essential to understand the aetiology of this infection which in turn helps create new strategies to control it. Thus, this study is focused on identifying the aetiological agents causing mastitis and its antibiotic susceptibility profiles in lactating buffaloes of a single herd in Selangor. A total of 50 lactating female buffaloes from a farm in Beranang, Selangor were selected for this study and detected for clinical mastitis (CM) and subclinical mastitis (SCM) infections using visual examination and a California Mastitis Test respectively. The mastitis infected buffalo milk was collected and sampled aseptically and subjected to bacterial culture. Gram-staining was done on the isolated bacterial colonies and were then subjected to Analytical Profile Index (API) tests according to their cell morphologies to determine the causative agents of SCM buffalo milk in this investigation. Antibiotic Susceptibility Test (AST)using Kirby-Bauer disk diffusion method was done on the bacterial isolates discovered using 10 types of antibiotics commonly used to treat bubaline mastitis. The results were recorded, calculated and analysed in Microsoft Excel Spreadsheet. The overall prevalence for buffaloes infected with CM was 0% as there were none present. Conversely, SCM was 70% prevalent. 143 bacterial isolates of 46 types of causative agents were identified from the SCM buffaloes. The most prevalent causative agent was Streptococcus agalactiae (14.7%). AST results revealed that, Gram-positive bacteria (GPB) were most susceptible to Amoxycillin/Clavulanate (98.2%), Erythromycin (92%), Enrofloxacin (92%) whilst Gram-negative bacteria (GNB) to Gentamicin (100%), Enrofloxacin (93.3%), Ceftriaxone (93.3%) in order. In contrast, GPB and GNB were most resistant against Streptomycin (42.5%) and Penicillin G (76.7%) respectively. Conclusively, this study provides a good understanding on the appropriate therapeutic regimes to be employed during bubaline mastitis infections.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 29 Jul 2024 03:15 |
| Last Modified: | 29 Jul 2024 03:15 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1962 |
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