The effect of feed-based vaccination against vibriosis on stimulation of the gut-associated lymphoid tissue (GALT) in Asian Seabass (lates calcarifer)

Romanus, Dwayne Norman (2022) The effect of feed-based vaccination against vibriosis on stimulation of the gut-associated lymphoid tissue (GALT) in Asian Seabass (lates calcarifer). [Project Paper] (Submitted)

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Abstract

Vibriosis is an important disease in cage-cultured fish worldwide, including Malaysia. Currently, vaccination is regarded as the way forward in controlling the disease. Vaccine administration via injection is laborious, thus we designed a feed-based vaccine from formalin-killed Vibrio harveyi. This study seeks to determine the effect of administration of this vaccine on gut-associated lymphoid tissue (GALT) in Asian Seabass in field setting. Archived paraffin blocks of gut samples from a previous study conducted in Pulau Ketam, Selangor, were used. The samples consisted of two groups; vaccinated, and non-vaccinated groups, which were sampled on week 0, 2, 4, 6, 8, and 10. Vaccination was administered to the vaccinated group on week 0, 2, and 4. Blocks from three fish per week for each group were sectioned, stained with haematoxylin and eosin and viewed under a light microscope to measure the area, lymphocyte number, and cell density of GALT in these groups. In general, both the control and vaccinated groups showed the presence of GALT starting from week 0 until week 10, where the GALT number was significantly (p < 0.05) higher in the vaccinated group than the control group throughout the course of study. Both groups showed no significant (p > 0.05) difference for the GALT area from week 0 until week 10. However, the lymphocyte numbers were significantly (p < 0.05) higher in the vaccinated group than control group between week 0 and week 10. Although both groups showed consistent increase in lymphocyte count throughout the study, the vaccinated group showed significantly (p < 0.05) higher GALT density than the control group throughout the study. Therefore, the feed-based V. harveyi vaccine elicited better GALT development in the vaccinated fish compared to non-vaccinated fish within the span of 10 weeks, thus resulting in a better protection rate against vibriosis.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	26 Jul 2024 03:42
Last Modified:	26 Jul 2024 03:42
URI:	http://psaspb.upm.edu.my/id/eprint/1987

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