Morphological and molecular identification of lymnaeid snails from selected buffalo farms under oil palm integration in Perak, Malaysia

Joganathan, Arvinderan (2022) Morphological and molecular identification of lymnaeid snails from selected buffalo farms under oil palm integration in Perak, Malaysia. [Project Paper] (Submitted)

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Abstract

The Lymnaeidae family of freshwater snails are of key interest in veterinary and human medicine worldwide due to being intermediate hosts of digenean trematodes such as Paramphistomum sp and Fasciola sp. Accurate identification of the intermediate host for these trematodes is key to understanding disease epidemiology and effective control. However, despite their essential role in the parasite’s life cycle, the research about freshwater snails identification and distribution in Malaysia is scarce with reliable information only available in neighboring countries. This study aimed to identify Lymnaeids in buffalo farms under oil palm integration in Perak through morphological and molecular approaches. A total of 106 freshwater snails were collected at waterlogged areas in two buffalo farms and the adjacent wetlands and marshes in Perak. Morphological characterization of the snail samples was done through analysis of seven morphometric parameters of their shells such as shell height (SH), shell width (SW), spire height (SpH), aperture width (AW), aperture height (AH), number of whorls (NoW) and orientation of shell (OS), in reference to a key and other literature for identification. Molecular analysis on the five selected samples using foot tissue for DNA extraction and the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA as molecular markers revealed that Lymnaeids collected were of the species Radix rubiginosa, a common intermediate host species for both Paramphistomum sp and Fasciola sp.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	26 Jul 2024 03:46
Last Modified:	26 Jul 2024 03:46
URI:	http://psaspb.upm.edu.my/id/eprint/1989

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