Ngah@Ibrahim, Hadibah (2015) The effect of different extraction method toward extracted (1,3;1,4)-β-glucan fine structure from rice grain, leaf and stem. [Project Paper] (Submitted)
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Abstract
The (1,3;1,4)- β-glucan is polysaccharide found in the cell wall of commercial cereal and grasses. It has been reported to accumulate highly in the barley and oat grain. Depend on the fine structure at the respective ( 1,3; 1,4)- β-glucan, certain type of (1,3;1,4)- β-glucan are more soluble. Higher concentration of alkali is needed to extract the less soluble ( 1,3; 1,4)- β-glucan as the streamlined method using aqueous buffer is only able to extract soluble ( 1,3; l ,4)- β-glucan. The different extraction method will give different effect toward the total extracted (1,3;1,4)- β-glucan content in different rice tissue. Two types of extraction buffer were used in this work: the potassium hydroxide and aqueous extraction method. Both methods are modified to permit the analysis of small amount of sample. The Sarawak rice cultivars yield different concentration of the ( 1,3; l ,4)- β-glucan using both method on different rice tissue. Stem has the highest concentration of(l,3;1,4)- β-glucan compare to other rice tissue. In grain, the highest concentration of (l,3;1,4)- β-glucan is 0.59 (% w/w) obtained at Bario banjal (Setiam). 0.848 (% w/w) of (1,3;1,4)- β-glucan is the highest concentration obtained at leaf of Ce/um ha/us stumbin while in stern, the concentration of 4.768 (% w/w) is obtained at Bario banjal (Setiam). All the highest concentration of (1,3; 1,4)- β-glucan is obtained through the KOH extraction method.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Agriculture and Food Sciences |
| Depositing User: | Mr. Azman Mohamad |
| Date Deposited: | 01 Oct 2024 00:36 |
| Last Modified: | 01 Oct 2024 00:36 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2006 |
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