Antibiotic susceptibility testing and molecular detection of methicillin-resistant staphylococcus aureus (MRSA) associated with bubaline subclinical mastitis

Dzahrin, Nur Dzuhaila Aina (2023) Antibiotic susceptibility testing and molecular detection of methicillin-resistant staphylococcus aureus (MRSA) associated with bubaline subclinical mastitis. [Project Paper] (Submitted)

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Abstract

Subclinical mastitis is one of the diseases that cause economic losses worldwide due to the reduction in milk yield and the high treatment costs for dairy buffaloes. Further to this, the emergence of methicillin-resistant Staphylococcus aureus (MRSA) in bubaline subclinical mastitis has been increasing. This is because excessive antimicrobial usage acts as mastitis treatment and management regimes as preventive measures. Nevertheless, there is still a lack of data on the occurrence of MRSA in subclinical mastitis in buffaloes in Malaysia. Thus, this study aims to evaluate the antibiotic resistance profiles of Staphylococcus aureus isolates, identify the molecular typing of MRSA isolates using conventional polymerase chain reaction (PCR), and identify the occurrence of MRSA in bubaline subclinical mastitis from selected farms in Selangor, Malaysia. A total of 32 archived samples of S. aureus originated from cases of bubaline subclinical mastitis from six different farms in Selangor were obtained and assessed for bacterial analysis. The samples were tested for antibiotic susceptibility testing (AST), and it was found that most showed resistance to cloxacillin (47%), penicillin (25%), and methicillin (15%). Common antibiotics used as mastitis treatment and drying-off treatment are less effective against the isolated bacteria. Based on the PCR assay targeting the mecA gene in MRSA, there is no discernible band to the positive control of MRSA, which implies there is a 0% incidence of MRSA detected in the archived samples of Staphylococcus aureus. The current results suggested that the dairy buffaloes from the selected farms were not infected with MRSA. However, it might suggest a false negative result due to the emergence of new variants of MRSA, mecC and PCR assays not targeting multiple primers, mecA and mecC simultaneously. Additionally, the gene of archived samples could be inactive due to gene mutation. Hence, detection of MRSA is important, as early prevention and control are needed considering the vast majority of MRSA are multidrug-resistant.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	15 Jul 2024 08:18
Last Modified:	15 Jul 2024 08:18
URI:	http://psaspb.upm.edu.my/id/eprint/2013

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