In vitro investigation of the effect of kyllinga brevifolia as a potential phytotherapy in feline parvovirus infection

Hashim, Hanizah (2023) In vitro investigation of the effect of kyllinga brevifolia as a potential phytotherapy in feline parvovirus infection. [Project Paper] (Submitted)

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Abstract

Feline panleukopenia, caused by feline parvovirus (FPV), is a disease that has a high morbidity and mortality rate in cats. FPV is a non-enveloped single-stranded DNA virus that is resistant and can persist in the environment for a long time. Currently, there is no treatment available for feline panleukopenia, causing pet owners in Malaysia to choose other alternatives, such as feeding their cats the shortleaf spikesedge plant, also known as Kyllinga brevifolia (KB), as they believe it would treat FPV infection. However, there is no scientific report stating the antiviral potential of KB in treating FPV infection. In order to investigate the validity of the claim, this study was carried out to evaluate the potential antiviral effect of KB in FPV infection. Cell titer blue assay was used to determine the cytotoxicity of KB on the Crandell-Rees Feline Kidney Cell (CRFK) cell line. FPV at the concentration of 1x105 TCID50 was inoculated in CRFK cell lines and treated with KB, whereas CRFK cells inoculated with PBS and FPV only served as negative and positive control, respectively for 48 hours at temperature 37°C with 5% CO2. Kyllinga Brevifolia at 25% concentration yields the highest percentage of cell viability (86%) after treatment. Positive control and KB-treated CRFK cells showed formation of CPE, but less CPE was observed in KB-treated cells. Additionally, moderate reduction of FPV titers was also observed in KB-treated cells to 1x103.5 TCID50. The finding shows that KB causes a reduction of CPE and viral titers on CRFK cell lines after infection with FPV, suggesting that it may have potential antiviral properties against feline parvovirus. However, further in vivo studies are required to confirm the antiviral properties of KB.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	30 Sep 2024 06:42
Last Modified:	30 Sep 2024 06:42
URI:	http://psaspb.upm.edu.my/id/eprint/2064

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