Bacterial isolation, identification and antimicrobial susceptibility testing of bubaline subclinical mastitis from selected farms in Selangor

Ahmad Julaihi, Kamelia (2023) Bacterial isolation, identification and antimicrobial susceptibility testing of bubaline subclinical mastitis from selected farms in Selangor. [Project Paper] (Submitted)

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Abstract

Subclinical mastitis is an insidious inflammation of the mammary gland that lacked in visible local or systemic symptoms and poses a significant threat to the dairy industry. This concern extends to the dairy buffalo sector, where research on this disease, particularly in Malaysia, has been lacking. Therefore, this study aims to address this gap by investigating the bacterial culprits behind subclinical mastitis in buffaloes, understanding the infection patterns, and assessing their antibiotic susceptibility profiles. Milk samples were collected from 18 lactating dairy buffaloes of the Murrah and Nilli-Ravi breeds from six selected farms in Selangor. A total of 72 milk samples were gathered from individual teats of these buffaloes, each of which underwent physical examinations and the California mastitis test. Bacterial identification began with assessing the colony and cell morphologies, Gram-staining, and biochemical traits of the isolated bacterial strains. Antibiotic susceptibility testing (AST) was then performed using ten antibiotics of choice namely enrofloxacin, erythromycin, ceftriaxone, gentamicin, trimethoprim, amoxicillin, penicillin G, streptomycin, tetracycline, and ampicillin. The antimicrobial susceptibility profiles of each bacterium were determined by interpreting their zones of inhibition based on Clinical Laboratory Standard Institute (CLSI). A total of 70 bacteria were isolated and identified, of which 63 (90%) were gram-positive bacteria and 7 (10%) were gram-negative bacteria. The most frequently encountered bacterium was coagulase-negative staphylococci isolates (n=26, 37.14%). Regarding antibiotic susceptibility, gram-positive bacteria (90.48%) exhibited higher susceptibility to gentamicin and amoxicillin/clavulanic acid, while gram-negative bacteria (100%) were most susceptible to trimethoprim-sulfamethoxazole. Conversely, both gram-positive (26.98%) and gram-negative bacteria (57.14%) displayed the highest resistance to streptomycin. This study enhances our knowledge of bacteria associated with bubaline subclinical mastitis and provides updated antibiotic susceptibility profiles. It underscores the need for improved farm management and prudent antibiotic use to combat mastitis in the dairy buffalo sector, contributing to the foundation for effective mastitis control in smallholder buffalo farming.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	30 Sep 2024 06:45
Last Modified:	30 Sep 2024 06:45
URI:	http://psaspb.upm.edu.my/id/eprint/2066

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