Antidepressant properties of Mitragyna speciosa in depressive disorders model in mice

Abdullah, Nurul Akmaryanti (2012) Antidepressant properties of Mitragyna speciosa in depressive disorders model in mice. [Project Paper] (Submitted)

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Abstract

Summary: Depression is a well known psychiatric disorder with a high mortality and morbidity rate. Moreover, the pharmacotherapy used in hospital today is not suitable for all patients and causes various side-effects. Thus, looking for alternative treatments with antidepressant effect and minimal side-effect is important. Mitragyna speciosa traditionally been used to alleviate pain, reducing coughing and to encounter fatigue. Its anti-fatigue effect has been well known and used to enhance physical performance. This study is designed to determine the possible antidepressant action of methanol-chloroform extract from Mitragyna speciosa (MCE) leaves in depressive disorders model in mice. 112 male Bulb/C mice were divided into 6 groups. Group 1 (n=7) (vehicle treated) was not exposed to any test. Group 2 (n=21) serves as negative control group (vehicle treated), group 3 (n=21) serve as positive control group and was treated with antidepressant drug, Amitriptyline Hydrochloride (dose=15mg/kg), group 4 (n=21) received low concentration of MCE (dose=10 mg/kg), group 5 (n=21) received medium concentration of MCE (dose=100 mg/kg) while group 6 (n=21) received high concentration of MCE (dose=100 mg/kg). Group 2 to 6 were subdivided into 3 subgroups (n=7). Each subgroups was submitted to either one of the 3 different tests (FST/TST/ locomotor activity evaluation). The antidepressant drug and the 3 dosages of MCE from Mitragyna speciosa were fed for 7 successive days by force –feedings. All the tests were conducted after 90 minutes of oral administrations on the 7th day. Immediately after test was conducted, tip of the mice tail was cut to obtain few drops of bloods. The bloods then were smeared on slide and stained with May Grunewald stain. Locomotors activity was conducted to prove that Mitragyna Speciosa methanol-chloroform extract is not a stimulant. All doses of Mitragyna Speciosa extract (10,30,100 mg/kg) significantly reduced the immobility times of mice on both FST and TST, without any significant effect on locomotors activity of mice. The efficacy of extract was found to be comparable to that Amitriptyline Hydrochloride (15 mg/kg). Analysis of variance (ANOVA) show the three concentration of MCE and positive drug show no significant different in neutrophil-lymphocyte ratio after to both FST and TST. This indicates that MCE show effect in normalizing the number of circulating neutrophils and lymphocytes in blood under appropriate level that similar to normal group. Thus, it is concluded that Mitragyna Speciosa methanol-chloroform extract may possess an antidepressant-like effect.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	22 Nov 2022 06:37
Last Modified:	15 Feb 2023 01:45
URI:	http://psaspb.upm.edu.my/id/eprint/208

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