Metabolic disorder and virus detection in organs of chicken following infection with fowl adenovirus serotype 8B

Mohd Dzilan, Muhammad Aidil Haikal (2023) Metabolic disorder and virus detection in organs of chicken following infection with fowl adenovirus serotype 8B. [Project Paper] (Submitted)

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Abstract

Fowl adenoviruses (FAdVs) are non-enveloped double-stranded DNA viruses and they are responsible for a variety of diseases in chickens including inclusion body hepatitis (IBH), hydropericardium hepatitis syndrome (HHS), and adenoviral gizzard erosion (AGE). The aim of study is to determine the metabolic parameters and virus detection in organs of chickens following infection with FAdV serotype 8b. Fifteen, day-old specific pathogen free (SPF) chicks were divided into two groups namely, infected group and control group. Six chicks were assigned in infected group and nine chicks in the control group. All chicks in the infected group were inoculated with 1ml FAdV isolate, UPM1901, at virus titer 107.1TCID50/ml via oral route at day 7 of age. All chicks in the control remained uninoculated in this study. All chicks were monitored or any clinical signs associated with IBH throughout the trial. During sampling, body weight, liver and bursa weight were measured including blood collection for serum biochemistry analysis at day 0 post-inoculation (pi) for control group and at day 3 and 7pi in both groups. Samples of liver, thymus, cecal tonsil, kidney, bursa of Fabricius, spleen, and bone marrow were collected for histological examination and polymerase chain reaction (PCR) analysis. It was demonstrated that infected chickens with FAdV were depressed, ruffled feathers and inappetence at day 6pi. Upon necropsy, swollen, haemorrhage and necrosis of liver was observed in chicken from infected group with FAdV at day 3pi. Liver weight of chicken for infected group was increased significantly (p<0.05) at day 3pi (5.3 ± 0.1) and day 7pi (5.6 ± 0.6) compared to the control group. In addition, liver to body weight ratio was also significantly higher (p<0.05) at day 3 and 7pi than the control group due to enlarged liver. Numerous basophilic intranuclear inclusion body (INIB) was observed in the hepatocytes at day 3 and 7pi of the infected group which is an indicator of viral particles exists in the liver organ. Serum biochemistry results showed liver enzymes for aspartate-aminotransferase (AST) and gamma-glutamyl transferase (GGT) level were elevated in chickens from FAdV infected group at day 3 and 7pi but lower creatine kinase (CK) level than control group probably due to liver injury caused by FAdV infection. FAdV was detected by PCR in liver, kidney and lymphoid organs at day 3 and 7pi in group A. It was confirmed that the FAdV isolate serotype 8b, UPM1901, induces metabolic disturbances in SPF chicken following inoculation at 7 days old with evidence of virus distribution in various organs.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Sep 2024 07:35
Last Modified:	30 Sep 2024 07:35
URI:	http://psaspb.upm.edu.my/id/eprint/2080

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