Anti-inflammatory and anti-pyretic effects of free and liposome-encapsulated piroxicam in rats

Mohd Hijaz Mohd Sani, Mohd Hijaz Mohd Sani (2010) Anti-inflammatory and anti-pyretic effects of free and liposome-encapsulated piroxicam in rats. [Project Paper] (Submitted)

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Abstract

Summary: Piroxicam is in a group of drugs called non-steroidal anti-inflammatory drugs (NSAIDs), which works by reducing hormones that cause inflammation and pain in the body. Liposome is lipid bilayers surrounding an aqueous space which can be use as a drug delivery system. This study is conducted to test the anti-inflammatory and anti-pyrectic efficacy level between free and liposome encapsulated piroxicam in rats. Liposome is prepared by mixing 1 gram of Prolipo compound with 200 µ1 of 60ng/m1 DMSO. After one hour of reaction, the mixture compound is hydrated with 1.8 ml of distilled water (dH₂O) for 10 hours. Then another 5 µ1 dH₂O added for 30 minutes to dilute the concentrated liposome. Preparation of all liposomal samples was carried out at room temperature. The animals were randomly divided into 2 groups of rats. The first group received free Piroxicam (Px) while the other received liposome-encapsulated piroxicam (LPx). Each group was further divided into 4 subgroups [0(control), 0.3, 3 and 30 mg/kg], with 6 animals each. In acute anti-inflammatory assay, male Sprague Dawley rats used were injected subcutaneously with 0.1 m1 of 1% carrageenan onto the plantar surface of right hind paw. The percentage of swelling for each group is calculated and compared. Cotton pellet weighing 30 mg, implanted aseptically at intrascapular distance under the skin on the shave backs of anaesthetized rats. The rats were sacrificed at the 8th day of experiment and the moist cotton obtained weighed, dried, and weighed again. The data obtained analysed to determine its transudative and granuloma weight inhibition. Hyperthermia was induced in rats by injected sub-cutaneously then with 10mg/kg of 20% aqueous suspension of Brewer’s yeast. The body temperature was measured at 1 hour intervals up to 5 hours after administration of drug. Anti-inflammatory assay shown LPx to exhibit greater therapeutic effect compared to Px. Group treated with 30 mg/kg LPx showed a significant swelling inhibition with a maximum of 90.5% at the final hour. In addition, LPx also shown to possessed anti-inflammatory effect at a lower dose. In chronic inflammation, result obtained shows that 3 and 30 mg/kg LPx produced a significantly better anti-inflammatory property compared to Px. LPx produced quicker and prolonged anti-pyretic effect at the highest dosage when compared to Px. Thus from the results obtained, it is concluded that the encapsulation of piroxicam by liposome enhanced the therapeuticeffect of the drug in both anti-inflammatory and anti-pyretic assays.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	12 Sep 2022 02:47
Last Modified:	21 Mar 2023 00:06
URI:	http://psaspb.upm.edu.my/id/eprint/209

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