Comparative evaluation of bull semen quality between ultra rapid freezing and slow freezing cryopreservation methods

Muhammad Rafidi, Muhammad Farid Imran (2023) Comparative evaluation of bull semen quality between ultra rapid freezing and slow freezing cryopreservation methods. [Project Paper] (Submitted)

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Abstract

Semen cryopreservation plays a pivotal role in preserving genetic material for future applications in animal husbandry, notably in cattle breeding for artificial insemination. This study investigates the quality of bull semen preserved through two distinct cryopreservation techniques: the conventional slow freezing method and the comparatively novel ultra-rapid freezing method. In this experiment, bull semen was collected using an electro ejaculator and subsequently diluted in a Tris-egg yolk extender containing Tris buffer, fructose, citric acid, glycerol with egg yolk and commercially readily available Andromed extender. The semen samples were divided into two aliquots for cryopreservation. The first aliquot followed the standard slow freezing protocol, involving gradual cooling at 5°C for 2 hours then arranged horizontally on a rack, 3-4 cm above the surface of liquid nitrogen gas for 10 minutes before being cryopreserved in liquid nitrogen for 24 hours. Conversely, the second aliquot underwent ultra-rapid freezing, with a quick cooling phase at 5°C for 30 minutes, followed by immediate placement of straws into liquid nitrogen for 24 hours. Subsequently, the straws were thawed at 37°C for 30 seconds, and semen motility was evaluated using computer-assisted semen analysis (CASA), while morphology and viability were assessed through eosin-nigrosin staining. The results of the frozen-thawed semen analysis for slow freezing using tris-egg yolk extender indicated a significant enhancement (p < 0.05) in sperm viability compared to ultra-rapid freezing using tris-egg yolk and Andromed extenders. However, there were no significant differences (p > 0.05) observed in terms of morphological abnormalities and progressive motility between the groups. In conclusion, ultra-rapid freezing may not be a suitable alternative for cryopreserving bull semen, as it did not yield substantial benefits in terms of viability, morphology and progressive motility.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Oct 2024 07:35
Last Modified:	30 Oct 2024 07:35
URI:	http://psaspb.upm.edu.my/id/eprint/2096

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