Effects of different fixative agents in prolonged fixation on histological structures of murine renal tissue

Samsuddin, Mastura (2023) Effects of different fixative agents in prolonged fixation on histological structures of murine renal tissue. [Project Paper] (Submitted)

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Abstract

The assessment of the kidney at the cellular level is required to determine the kidney’s health status. Thus, preparing the kidney for histological examination is critical. The fixation step is extremely important since the solutions used and fixation durations determine the tissue quality examined during the viewing step. Due to the large caseload in the histopathology laboratory, many research projects require prolonged fixation and preservation of specimens. Thus, the current study intends to examine and compare the effects of different fixative agents on murine renal histological evaluation under prolonged fixation. The sections of renal tissue were taken from archived samples of the specific pathogen-free murine. The following fixatives were used: 10% Neutral-Buffered Formalin (NBF), 10% Formalin, 4% Paraformaldehyde (PFA), 10% NBF followed by 70% Ethanol (EtOH), and 10% NBF followed by Phosphate-Buffered solution (PBS). Hematoxylin and eosin (H&E) were used for microscopic examination of the processed tissues. Histological structures observed were renal corpuscles, proximal convoluted tubule, distal convoluted tubule, loop of Henle, and collecting duct. Under 40X magnification, renal histology was evaluated by the percentage of autolysed cells. Sections fixed with 10% NBF followed by 70% Ethanol provided acceptable preservation of tissue morphology as they had the lowest percentage of autolysed cells (38%) under prolonged fixation. Sections fixed with 10% Formalin had the highest percentage of autolyses (48%). The other sections fixed with 10% NBF, 10% NBF followed by PBS, and 4% PFA have 40%, 44%, and 47% of autolyses respectively. Overall, 10% NBF followed by 70% EtOH proved to be the best fixative for prolonged fixation as it resulted in the least effects on cellular histology compared with other aldehyde-based fixatives.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	15 Nov 2024 02:01
Last Modified:	15 Nov 2024 02:01
URI:	http://psaspb.upm.edu.my/id/eprint/2114

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