Detection of papillomavirus in squamous cell carcinoma in cats

Ng, Yen Dee (2023) Detection of papillomavirus in squamous cell carcinoma in cats. [Project Paper] (Submitted)

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Abstract

Human papillomaviruses (PVs) are known to cause squamous cell carcinoma (SCC) of the mouth and genitalia. In non-human mammals, there is increasing evidence indicating that PVs may play a causal role in the development of SCC in species like rabbits, dogs, and cats, particularly cutaneous SCC in cats, however, the exact role of PV in the development of SCC in cats remains unclear as investigations into its carcinogenic mechanisms remain scarce. Furthermore, the prevalence of PV in feline SCC (fSCC) in Malaysia is still unknown. This study aims to detect the presence of PV in feline oral and cutaneous SCC where 35 fSCC paraffin block samples were collected from the Histopathology Laboratory of the Faculty of Veterinary Medicine, Universiti Putra Malaysia between 2017 and 2023. The gender, age and location of mass were recorded. Immunohistochemistry was conducted to detect the PV in the fSCC samples using anti-HPV and anti-p16CDKN2A (p16) antibodies after evaluation of haematoxylin and eosin sections. The association between gender, age, and location of SCC with the detection of PV was analysed using either Chi-square test or Fisher’s exact test in SPSS. Positive p16 immunoreactivity was detected in 16 out of 35 samples (45.7%) whereas all 35 samples showed negative immunoreactivity for anti-Human Papillomavirus (anti-HPV) antibody (100%). There was an association between p16 overexpression and gender (p<0.05), with strong p16 immunoreactivity observed in SCC from male cats. However, neither the age (p=0.462) nor the location of SCC (p=0.082) was significantly associated with p16 overexpression. In conclusion, the overexpression of p16 in a subset of fSCCs suggests that there could be an association between PVs and the development of fSCC although its underlying molecular mechanisms that contribute to feline squamous cell tumourigenesis remains to be elucidated. Keywords:

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	15 Nov 2024 01:50
Last Modified:	15 Nov 2024 01:50
URI:	http://psaspb.upm.edu.my/id/eprint/2116

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