Relationship of milking practices and milk microbial diversity with prevalence of subclinical mastitis in lactating dairy buffaloes in Selangor, Malaysia

Ramdan, Rusyaidi Shah (2023) Relationship of milking practices and milk microbial diversity with prevalence of subclinical mastitis in lactating dairy buffaloes in Selangor, Malaysia. [Project Paper] (Submitted)

Text lp fpv 2023 38.pdf Download (1MB)

Abstract

Mastitis is one of the most serious diseases affecting dairy producers globally. Even for buffalo species, mastitis remains the dairy industry's most expensive disease, despite the traditional belief that buffaloes are less susceptible to mastitis than cattle. Subclinical mastitis is distinguished by an increase in somatic cell count and presence of bacteria in milk, but no physical abnormalities on the udder or in the milk. The purpose of this study is to assess the relationship of milking practices and bacterial profiles with the prevalence of subclinical mastitis of dairy buffaloes in Selangor, Malaysia. This study was conducted at six semi-intensive buffalo farms in Selangor, with 46 lactating buffaloes identified. The California Mastitis Test (CMT) was used to identify buffaloes with subclinical mastitis. Eighteen out of 46 lactating buffaloes, equivalent to (39.13%), were found to have subclinical mastitis. All positive samples were subjected to bacterial isolation and identification, yielding a total of 69 microorganisms, with 19 types of bacteria identified using a biochemical test. The most common bacteria discovered were coagulase negative staphylococci (CoNS) (27.54%), followed by Staphylococcus intermedius (17.39%), Streptococcus agalactiae (10.14%), Staphylococcus aureus (8.70%), Corynebacterium diphteriae (7.25%), and Corynebacterium spp. (4.35%). Other bacteria detected, such as Streptococcus dysgalactiae, Klebsiella spp., Acinetobacter iwoffii, and Enterococcus spp., were determined to be less than two percent. Among all four criteria of the good milking practices observed in all farms, milking technique showed a strong significant relationship with the number of pathogenic bacteria isolated in subclinical mastitis and prevalence of subclinical mastitis on each farm. Conclusively, CoNS is the most common pathogens causing subclinical mastitis and most lactating dairy buffaloes had high prevalence of subclinical mastitis. The significant relationship observed between milking technique with prevalence of subclinical mastitis and number of bacteria isolated in the milk highlights the critical importance of employing proper milking practices to safeguard buffalo udder health and maintain high milk quality.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	19 Nov 2024 02:40
Last Modified:	19 Nov 2024 02:40
URI:	http://psaspb.upm.edu.my/id/eprint/2164

Actions (login required)

View Item