Production of sugar from soya bean waste (OKARA) by enzymatic hydrolysis.

Lim, Amy Jia Hui (2018) Production of sugar from soya bean waste (OKARA) by enzymatic hydrolysis. [Project Paper] (Submitted)

Text FK 2018 26.pdf Download (59MB)

Abstract

In soya milk processing, large amount of soya bean waste known as okara can be utilize to recover sugar. The sugar can be recovered using enzymatic hydrolysis as one of the effective method due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of enzyme concentration and hydrolysis temperature on sugar production using enzymatic hydrolysis. Soya bean waste, a residue from soya milk production will act as the substrate was hydrolyzed by enzyme alpha amylase to produce sugar. The monosaccharide or oligosaccharide that hydrolyzed by the enzyme alpha amylase was determined by high performance liquid chromatography (HPLC). From the HPLC result showed that soya bean waste sample contains oligosaccharide which is sucrose and monosaccharide which is arabinose. The optimal condition chosen by the central composite design is hydrolysis temperature of 60° C with enzyme concentration of 2%. At 60°C with enzyme concentration of 2%, enzyme alpha amylase able to hydrolyze the total carbohydrate yield of 1831.00 mg/L and reducing sugar yield of 837.50 mg/L. By changing the enzyme concentration and hydrolysis temperature will results in different sugar yield production because the enzyme lost their three-dimensional functional shape due to disruption from unstable conditions of the environment system. As a conclusion, all the requirements for complementary in the configuration of soya bean waste and enzyme must be considered seriously for the highest sugar yield production.

Item Type:	Project Paper
Faculty:	Faculty of Engineering
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	14 Nov 2024 01:20
Last Modified:	14 Nov 2024 01:20
URI:	http://psaspb.upm.edu.my/id/eprint/2234

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