Cytological analysis on oil palm transgenic chromosomes

Mustafa, Abdul Razak (2007) Cytological analysis on oil palm transgenic chromosomes. [Project Paper] (Submitted)

Text lp FSPM 2007 1.pdf Download (13MB)

Abstract

The main aim of this research project is to perform karyotyping procedure and to calculate significant differences between pairs of homologous chromosomes in order to produce a karyogram of transgenic oil palm (TE 3-11) which is a plantlet that was modified through particle bombardment. Modifying genetic sequence might lead to chromosomes changes physically and also abnormalities during process of mitosis such as bridging and lagging. Pretreatment of the root tips using 2mM hydroxyquinoline increases the metaphase index of the cells hence karyotyping using Feulgen staining can be performed. Through microscope, these samples were observed, the images were captured and size measurements were made. Karyogram, a standardized arrangement of all the chromosomes described in term of its appearance at metaphase was produced. This research project used T-test to determine any significant differences between pairs of homologous chromosomes before arranging it. By using this statistical analysis, we found that chromosomes length varied from 1.057 - 4.155 microns and the mitotic activities were normal without any aberrations. This study concluded with a summary of the length similarities between 14 pairs of chromosomes on Elaeis guineensis Jacq. but differences with only 2 pairs of chromosomes (p<0.05).

Item Type:	Project Paper
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	17 Dec 2024 02:27
Last Modified:	17 Dec 2024 02:27
URI:	http://psaspb.upm.edu.my/id/eprint/2255

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