Mutagenicity assessment of effluent from Hospital Sultan Abdul Aziz Shah (HSAAS) using Ames test

Anak Timah, Bayliana (2024) Mutagenicity assessment of effluent from Hospital Sultan Abdul Aziz Shah (HSAAS) using Ames test. [Project Paper] (Submitted)

Text 208478_BAYLIANA AK TIMAH lp fpsk4 2024 48.pdf Download (4MB)

Abstract

Introduction: The increase amount of effluent produced by healthcare facilities everyday including hospitals, resulting a concern for environmental and public health. Hospital effluent consists of a complex mixture of hazardous contaminants which can be classified into physical remaining, chemical substances, biological, and organic risks that may induce mutagenic activity. Objectives: This cross-sectional study aimed to evaluate the mutagenic potential of Hospital Sultan Abdul Aziz Shah (HSAAS)’s effluent using Ames test. Methodology: The duration of study was from 1st September 2023 till 30th September 2023. About 250 mL samples were taken from five different sampling points at HSAAS in duplicate form namely grit chamber, aeration tank, clarifier tank, disinfection chamber, and discharge outlet to retention pond. Mutagenic activity was assessed using two types of Salmonella typhimurium strains (TA98 and TA100) with and without the presence of S9 metabolic activation system. Positive and negative controls were used for quality control of the experiment. Results: From five different samples, the number of revertant were higher in Salmonella typhimurium TA 98 (135 ± 68) as compared to Salmonella typhimurium TA 100 (85 ± 51) without the presence of S9 metabolic activation system. Sample from grit chamber was showed positive mutagenic (203 ± 23) as compared to control group in both Salmonella typhimurium TA 98 and TA100 without the presence of S9 metabolic activation (p<0.05). Meanwhile, the results with the presence of S9 metabolic activation system showed positive mutagenic activity in TA98 bacterial strains (p<0.05) but showed negative mutagenic activity in TA100 bacterial strains (p>0.05). It was showed that effluent treated with and without S9 showed a significant different for different bacterial strain (p<0.05). Conclusion: The hospital effluent showed a positive mutagenic in Salmonella typhimurium TA 98 and TA100 without S9 treatment. These findings emphasize the need for robust water quality monitoring to mitigate potential health and environmental risks, emphasizing the significance of effective management strategies. Keywords: Hospital effluent, mutagenicity, metabolic activation S9, Ames test

Item Type:	Project Paper
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	30 Apr 2025 02:31
Last Modified:	16 Oct 2025 01:23
URI:	http://psaspb.upm.edu.my/id/eprint/2399

Actions (login required)

View Item