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Culture medium optimization for selected phytoplankton species (Chlorella, Scenedesmus and Monoraphidium)

Veerappan, Shaktiroobini (2024) Culture medium optimization for selected phytoplankton species (Chlorella, Scenedesmus and Monoraphidium). [Project Paper] (Submitted)

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Abstract

Phytoplankton, having been the primary producers within aquatic ecosystems, have significant potential for a wide range of applications, that aim to enhance their growth rates and biomass production for potential applications in various industries, including aquaculture and biofuel production. Moreover, this study focuses on the optimization of culture media for three prominent phytoplankton species: Chlorella, Scenedesmus, and Monoraphidium comparing the widely used f/2 medium and BG-11 medium. This study aims to identify the optimal culture medium that will foster the growth of these microalgae. To study the effects of different both f/2 and BG-11 medium on the growth responses of the chosen phytoplankton species, a systematic approach is utilized. Moreover, the results indicate distinct Chlorella, Scenedesmus, and Monoraphidium responses to the different culture mediums on their specific nutritional requirements. A comparative analysis examined the differences in growth between f/2 and BG-11 medium. The study aims to identify which medium best suits the specific nutritional needs of each microalgae. This information is crucial for optimizing cultivation practices and achieving higher biomass production. Other than that, understanding the specific nutritional requirements of microalgae can lead to more resource-efficient cultivation. By using the most suitable culture medium for each species, can minimize the use of costly nutrients and reduce the environmental impact associated with microalgae cultivation. Thus, the results show, that these three species grow well in both mediums. Thus, Chlorella thrives in both media, as indicated by its quick and constant development at all time periods. However, Scenedesmus exhibits significant expansion in the BG-11 medium, indicating that this medium is especially well-suited for sustaining its growth. While both media stimulate the growth of Monoraphidium, the data indicates that BG-11 may be more favourable for achieving a faster growth rate in the current case. In summary, the study finds that f/2 medium performs better in terms of growth and biomass output, making it more suitable for the production of green algae. However, BG-11 medium has the potential for large-scale production even if it isn't the best for the particular green algae but the formulation and nutrient profile may be better suited for larger-scale cultivation.

Item Type: Project Paper
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Faculty: Faculty of Agriculture and Forestry Sciences
Depositing User: Mr. Azman Mohamad
Date Deposited: 08 Dec 2025 08:27
Last Modified: 08 Dec 2025 08:27
URI: http://psaspb.upm.edu.my/id/eprint/2541

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