Association between PM10 and PM2.5 with DNA damage in exfoliated Buccal mucosa cell among primary school children living near palm oil activity at Semenyih, Selangor

Othman, Syhidatul Farhana (2019) Association between PM10 and PM2.5 with DNA damage in exfoliated Buccal mucosa cell among primary school children living near palm oil activity at Semenyih, Selangor. [Project Paper] (Submitted)

Text FPSK4 2019 23 COMPLETED REPAIR.pdf Download (126MB)

Abstract

Introduction: The combustion of biomass material such as fibre and shell of fresh fruit bnmch will release particulate matters PM10, PM2.5, carbon monoxide, nitrogen oxide, and sulphur dioxide which contributes to air pollution problem. Exposure of PM2.5 released from combustion of biomass activity caused respiratory health among p1imary school children. Objective: This research aimed to determine the association between exposure of PM2.5 and PM10 with DNA damage in exfoliated buccal mucosa cells among primary school children living nearby palm oil combustion activity at Semenyih. Methodology: The study was conducted at two different primary schools where Sekolah Kebangsaan A was chosen as studied group and Sekolah Kebangsaan B as a comparative group. Those schools were selected based on the distance of school from palm oil factory. A total of 167 primary school children aged 9-11 years old were randomly selected from the list name for both groups to participate in this study. A standardized questionnaire was distributed to respondent's parents. Concentrations of PM2.5 and PM10 were collected by using Dust Trak DRX Aerosol Monitor Model 8534 and Escort LC Personal Sampling Pump. Measurement of indoor and outdoor air pollutants were conducted in schools and home. Buccal mucosa cells were collected, which then followed by micronucleus assay. Result: Mean±SD concentration of PM10, (104.29±58.70µg/m3) among studied group were significantly higher compared to comparative group, (29.29±16.69 µg/m3); (p=0.07). PM2.s at homes of studied group was higher (74.29±42.57 µg/m3) compared to comparative group with (Median±IQR) (28.57±32.11 µg/m3); (p=0.018). PM10 and PM2.s of studied schools were significantly higher (105.21±46.11); (68.83±13.03) compared to comparative schools (53.92 ±12.37); (50.80 ±17.45). MN frequencies of studied group were significantly higher (Median±IQR) (4.00±3.00) compared to comparative group (0.50±1.00); (p=0.001). Discussion: There were significant difference of respiratory symptoms between two groups of respondents with cough, phlegm, wheezing and chest tightness as p=0.001. There were significant correlation between PM10 with MN frequency of studied group and comparative group which indicated by r= 0.562; p=0.001. Conclusion: This research indicated that the exposure of PM10 and PM2.5 would increase the risk of having respiratory health symptoms among primary school children who lived near palm oil activity area. In the other hand, the exposure of particulate matters might induce the micronuclei formation among affected children. Lastly, this study would contribute to the baseline data on air quality and the health effects of primary school children based on frequency of micronuclei and respiratory health symptoms. Keywords: Primary school, Children, Particulate matters, Respiratory health, Micronuclei Frequency (MN)

Item Type:	Project Paper
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	29 Apr 2026 02:17
Last Modified:	29 Apr 2026 02:17
URI:	http://psaspb.upm.edu.my/id/eprint/2800

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