Effect of methanol extract of melastoma malabathricum (MEMM) leaves on carbon tetrachloride- and paracetamol- induced liver toxicity in rats

Kamisan., Farah Hidayah. (2011) Effect of methanol extract of melastoma malabathricum (MEMM) leaves on carbon tetrachloride- and paracetamol- induced liver toxicity in rats. [Project Paper] (Submitted)

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Abstract

Summary: In the modern system of medicine, there is no specific cure for a wide spectrum of liver toxicity. Thus, attempts are continuously being made to find out alternative agents from natural products sources for the treatment of hepatotoxicity. One of the plants that are being studied in our laboratory is Melastoma malabathricum, locally known as “senduduk”. Therefore, the present study aimed to evaluate hepatoprotective effect of MEMM against CCl4 – and PCM- induced liver toxicity in rats. For this study, 6 groups (n=6) of rats (Sprague Dawley; male; 180-200g) were used and received orally test solutions; 10% dimethyl sulfoxide (DMSO), 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg) once daily for 7 consecutive days. On the last day, 3 hours after the test solutions administration, hepatotoxicity was induced with CCl4 (1.5 ml/kg: intraperitoneally) and PCM (3 g/kg: orally). 48 hours after inducer administration, blood collection was carried out to obtain serum for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) tests then immediately the animals were sacrificed to obtain liver for histopathological examination. As the results, the groups pretreated with MEMM at 250 and 500 mg/kg significantly (P<0.05) reduced the elevated serum levels of AST and ALT in CCl4-treated group (negative control group). Based on histological examination, the negative control group has extensive interface hepatitis, multiple foci necrosis and haemorrhage of the hepatocytes. Interestingly, the groups pretreated with MEMM showed less severity of necrosis and inflammation of the hepatocytes compared to the negative control group. The pre-treated groups with MEMM showed insignificantly reduced in AST and ALT levels for PCM-treated group at dose 50 mg/kg, 250 mg/kg and 500 mg/kg. The mean score obtain for PCM-induced group showed significantly reduced at 50 mg/kg, 250 mg/kg and 500 mg/kg. As a conclusion, MEMM may possess hepatoprotective activity and further studies should be carried out to determine the exact dose that most effective for its hepatoprotective activity.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	22 Nov 2022 08:19
Last Modified:	15 Feb 2023 00:54
URI:	http://psaspb.upm.edu.my/id/eprint/281

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