Identification of potential bacterial pathogen in laboratory rats produced locally

Haron, Nur Asyikin (2015) Identification of potential bacterial pathogen in laboratory rats produced locally. [Project Paper] (Submitted)

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Abstract

Microbiological status of laboratory rats used in experiments can significantly influence the animals’ welfare and compromise research findings. Laboratory rats used in research must be healthy since any infection even in the absence of clinical signs before or during the experiment period may result in inaccurate data. In this study, 15 laboratory rats were chosen randomly from 3 different suppliers (5 rats from each supplier). The rats were euthanized and then, samples from the respiratory tract and gastrointestinal tract were collected using sterile swabs. Isolation and identification of bacteria from the samples were carried out by culturing followed by series of biochemical tests. Ten bacterial species were monitored in this study. They include Staphylococcus aureus, Bordetella bronchoseptica, Pasteurella pneumotropica, Streptococcus pneumonia, Corynebacterium sp., Salmonella sp., Citrobacter sp., Clostridium sp., Pseudomonas aeruginosa and Klebsiella pneumoniae. A number of bacterial species were identified, however, from the list mentioned, only Pasteurella pneumotropica, Corynebacterium sp., Pseudomonas aeruginosa and Klebsiella pneumoniae were present from the samples. These bacteria are known as opportunistic pathogens where immunocompromised animals may succumb to infection. Animals used in research will undergo stressful conditions during experimental period which may eventually lead to immunosupression and thus presence of these opportunistic pathogens may compromise the research data.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	21 Jun 2022 01:38
Last Modified:	22 Jun 2022 05:56
URI:	http://psaspb.upm.edu.my/id/eprint/308

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