Pathogenicity of fowl adenovirus isolates of Malaysia in specific pathogen free chickens

Ahmad, Majdi (2015) Pathogenicity of fowl adenovirus isolates of Malaysia in specific pathogen free chickens. [Project Paper] (Submitted)

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Abstract

Fowl Adenovirus (FAdV) is a non-enveloped virus and is a primary pathogen to cause inclusion body hepatits (IBH) in chickens. Affected chicken showed high mortality with typically lesions of pale, friable, haemorrhage and enlarged liver, and the present of intranuclear inclusion bodies (INIB) in the organ. It was the objective of the study to determine the pathogenicity of recent FAdV isolates of Malaysia in specific pathogen free (SPF) chickens. Thirty six one-day-old SPF chicks were divided into 3 major groups namely groups A, B and C. The chickens were then divided into sub-group namely the sacrificed and mortality groups. Groups A and B were inoculated (0.5mL/chick) with FAdV isolates A (UPM11134) and B (UPM1127), respectively via intra-peritoneal route. The group C was acted as a control group and remained un-inoculated. The chicks were observed at least twice daily for any clinical abnormality and mortality. Samples of liver, spleen, gizzard and trachea were collected and fixed in 10% buffered formalin for histological examination. The study showed a 100% mortality in the mortality groups A and B within 3 and 4 days post-inoculation (pi), respectively. While in the sacrificed groups A and B, mortality of 100% and 90% respectively were recorded within 4 and 7 days pi. The control group remained no mortality throughout the whole period. On necropsy, gross lesions of enlarged and yellowish liver with petechial hemorrhages were recorded in chicks from groups A and B. The spleen was enlarged and yellowish, whilst no significant lesions were recorded in the gizzard and trachea. Histological examination of the liver and gizzard from groups A and B revealed typical basophilic intranuclear inclusion bodies. No significant lesions were recorded in the trachea and spleen. It was concluded that FAdV isolates of Malaysia is highly pathogenic to one-day-old SPF chicks following inoculation of the virus via intra-peritoneal route.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	16 Nov 2022 01:03
Last Modified:	16 Nov 2022 01:03
URI:	http://psaspb.upm.edu.my/id/eprint/325

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