Efficacy of porcine circovirus vaccine in reducing viraemia status in pigs

Sia, Bang Wen (2015) Efficacy of porcine circovirus vaccine in reducing viraemia status in pigs. [Project Paper] (Submitted)

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Abstract

Post-weaning multisystemic wasting syndrome (PMWS) in pigs is a disease associated with porcine circovirus type 2 (PCV2) infections. In 2007, sporadic cases of PMWS had caused major economic losses in Malaysia swine industry. PCV2 vaccines were suggested as an important strategy in the prevention of PMWS. Thus, field trial of PCV2 vaccine was performed in order to evaluate the efficacy of the vaccine in reducing the PCV2 viraemia in pigs. Thirty piglets of three weeks old were randomly selected from 5 litters and equally divided into two groups of 15 pigs (treatment and control group). The treatment group was administered with 2 mL of PCV2 vaccine intramuscularly. Blood samples and body weight were obtained on days 0 (pre-vaccination), 20 and 40 post-vaccination. Samples were tested using quantitative PCR (qPCR) assay to evaluate the viral load in blood. This study revealed that on day 40 post-vaccination, vaccinated pigs showed lower PCV2 viral DNA copies number (5.01 log10 copies/mL of serum) compared to control pigs (6.18 log10 copies/mL of serum). One pig was sacrificed from each treatment and control groups for post-mortem examination at day 40 and it revealed no significant findings on lymphoid tissues from both groups. In addition to that, results on average body weight showed no significant difference between groups. This study suggested that PCV2 vaccine able to reduce PCV2 viraemia in vaccinated pigs.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	16 Nov 2022 01:17
Last Modified:	16 Nov 2022 01:21
URI:	http://psaspb.upm.edu.my/id/eprint/334

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