The ameliorative effects of Nigella sativa on the oxidative status and the pathology of Streptococcus agalactiae infection in red hybrid Tilapia (Oreochromis sp.)

Rahamat@Rahmat, Nurakmaliah (2015) The ameliorative effects of Nigella sativa on the oxidative status and the pathology of Streptococcus agalactiae infection in red hybrid Tilapia (Oreochromis sp.). [Project Paper] (Submitted)

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Abstract

Nigella sativa is a natural plant that could be used as antioxidant in addition to its prophylactic usage. Streptococcosis on the hand has become a global challenge to aquaculture industry all over the world. This study aimed at investigating the influence of Nigella sativa on oxidative stress and the pathology of Streptococcus agalactiae infection in Red hybrid tilapia. Hundred twenty fish weighing 50 to 150 g were randomly divided into four groups, A, B, C and D of 20 animals each with replicates. Group A was negative control while B was positive control in which both were fed with 100 % commercial diet. Group C and D were treatment groups fed with commercial diet incorporated with 8 % and 15 % N. sativa for 14 days, respectively. At the end of experiment, all the fish in group B, C and D were challenged with S. agalactiae, 107 CFU/mL at the rate of 100 μL, intraperitoneally. Blood and tissue sampling were taken at day 7, 14, 17, and 18 post-feeding. Plasma was harvested and used for malondialdehyde (MDA) while haemolysates were used for superoxidase dismutase (SOD) analysis as stress biomarkers. Statistical analysis from the value of MDA and SOD indicated that there were significant different between controls and treatments’ group. Histopathology examination at 18 hours post-infection (day 18 post-feeding) revealed significant findings between the groups. The incorporation of N. sativa in fish feed could ameliorate the stress and minimize the mortalities caused by streptococcosis.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	23 Nov 2022 04:17
Last Modified:	23 Nov 2022 04:17
URI:	http://psaspb.upm.edu.my/id/eprint/337

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