The anti-cancer activities of Salmonella enterica Serovar Agona in canine mammary gland tumour cells in vitro

Lee, Yee Wen (2015) The anti-cancer activities of Salmonella enterica Serovar Agona in canine mammary gland tumour cells in vitro. [Project Paper] (Submitted)

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Abstract

Canine mammary tumour is the most common neoplasm in female dogs which possesses epidemiological, clinicopathological and biochemical characteristics similar to human breast carcinoma. Previous studies showed that bacteria have anti-cancer properties. One of the commonly investigated bacteria as anti-cancer agent is Salmonella Typhimurium. In order to ensure safety of such in vivo therapy, the virulence of the bacteria must be attenuated to reduce the pathogenic effects. Recently Salmonella Agona, wild type strain (WA32) was genetically engineered to silence the metabolite and virulence genes to develop a genetically modified/ attenuated strain (4KA32) for cancer therapy. In this study, real-time cell analysis (RTCA) bacterial invasion assay was conducted in a canine mammary tumour cell line (CMT-Stylo) to evaluate the cytotoxicity of WA32 and 4KA32 bacteria in comparison with S. Typhimurium and doxorubicin. The CMT-Stylo cells were grown in E-plates for 24 hours and then treated with three concentrations of 104, 105, 106 CFU bacteria or control (media or doxorubicin). Real-time data on impedance measurement, expressed as cell index (CI), a parameter of cell viability, was recorded over 48 hours of treatment. Exposure of CMT-Stylo cells to different concentrations of WA32 and 4KA32 bacteria produced significant (p<0.05) cytotoxicity and 60% to 80% reduction in e cell index, compared to untreated control, and up to 90% compared to doxorubicin-treated cells. No significant (p>0.05) difference in CMT-Stylo cell cytotoxicity was observed towards treatment with any of the WA2 or 4KA32 bacteria concentrations. Microscopic examination revealed cell round-up in the treatment groups at 48 hours, which could possibly be due to apoptosis. In conclusion, this is the first report demonstrating that both attenuated and wild type S. Agona strains have significant cytotoxic effects on the canine mammary tumour cell line, CMT-Stylo. Since the attenuated strain produced similar cytotoxicity compared to the wild-type, the attenuated bacteria is recommended for anti-cancer therapy, with the assumption that it has minimal in vivo pathogenic effect. It is recommended that studies on laboratory animal and dog with spontaneous mammary tumours be conducted in the future.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	16 Nov 2022 03:57
Last Modified:	16 Nov 2022 03:57
URI:	http://psaspb.upm.edu.my/id/eprint/351

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