Zamzuri, Khairul Faizi (2011) Antidiabetic effect of polypeptide-k and essential oil from momordica charantia. [Project Paper] (Submitted)
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Abstract
Summary: Background: Momordica charantia known as bitter gourd is one of the useful herbs in traditional medicine especially in antidiabetic properties. Antidiabetic effects of bitter gourd extracts have been demonstrated in cell culture, animal, and human studies which can stimulate pancreatic insulin secretion and peripheral glucose metabolism, including stimulation of glucose uptake and inhibit gluconeogenesis. In this study, we investigated the effect of essential oil and polypeptide-k from extraction of Momordica charantia on α-glucosidase and α-amylase activity. Hypothesis: Polypeptide-k and essential oil has antidiabetic properties by inhibit α-glycosidase and α-amylase activity. Objectives: To assess the effect of polypeptide-k and essential oil for antidiabetic properties by assayed the α-glycosidase and α-amylase activity. Material and method: The essential oil and polypeptide-k were assayed for inhibition of α-amylase and α-glycosidase activity by using Alpha-Amylase Assayed Kit and Alpha-Glucosidase Assayed Kit respectively. Initial dosage of two samples is 2.0 mg/ml and undergoes serial dilution with ¾ dilution factor to reduce the concentration. 0.5ul from each sample and product of serial dilution will added to 0.5ul of alpha-amylase enzyme and alpha-glucosidase enzyme. Then, the mixtures were tested by using the kit and absorbance readings were recorded at 595nm for α-glycosidase assay and 405 nm for α-amylase assay. Result: From this study, polypeptide-k and essential oil from extraction of Momordica charantia has antidiabetic properties by showed significant α-glycosidase and α-amylase inhibitory activities. Different dosage has different inhibitory activity and the highest inhibitory activities are from the highest dosage of samples by showed the most effective and significant (p<0.0005) result of inhibition activity followed by lower concentration. Polypeptide-k has strong and effective inhibitory effect to α-glycosidase and α-amylase activity than essential oil by showing a high rate of inhibition activity by the equivalent of dosage use.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms Norafizah Radzuan |
| Date Deposited: | 23 Nov 2022 00:45 |
| Last Modified: | 15 Feb 2023 00:53 |
| URI: | http://psaspb.upm.edu.my/id/eprint/354 |
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