Effcet of aqueous extract to of melastoma malabathricum (AEMM) leaves on carbon tetrachloride- and paracetamol-induced lever toxicity in rats

Yahya, Farhana (2011) Effcet of aqueous extract to of melastoma malabathricum (AEMM) leaves on carbon tetrachloride- and paracetamol-induced lever toxicity in rats. [Project Paper] (Submitted)

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Abstract

Liver diseases have been associated with various factors, including continuous consumption of certain drugs and accidental exposure to certain toxic chemicals. Therapies of certain diseases developed by the Western medicine principles mainly associated with limited efficacy, have adverse effects, particularly liver diseases, and costly. Therefore, researchers have shifted their focus towards finding alternative for curing liver diseases, in particular, from natural sources, which include plant-based products. One of the plants that are currently being investigated in our laboratory is Melastoma malabatricum, locally known as ‘senduduk’, which has been reported to possess antinociceptive, anti-inflammatory, antioxidant and cytotoxic activities. In an attempt to further establish the pharmacological properties of M. malabathricum, the hepatoprotective potential of its leaf had been investigated using the CCl4- and PCM-induced liver toxicity in rats in this study. For this present study, the dried, grounded leaves of M. malabathricum (40g) were soaked with 800ml water (1:20 (w/v)) for 72h at room temperature, then filtered and subjected to the freeze-drying process. 11 groups (n=6) of rats (Sprague Dawley; male; 180-200g) were used in the present study and received orally distilled water, 200 mg/kg silymarin or AEMM (50, 200 and 500 mg/kg) once daily for 7 consecutive days. Hepatotoxicity was induced by administration of CCl4- (0.15 ml/kg) or PCM (3 g/kg) 3 hours after the last extract administration. On the 9th day, the animals were sacrificed and the liver was collected for histopathological examination. From the histological observation, hepatic steatosis, lymphocyte infiltration and marked necrosis was observed in CCl4 and PCM- treated groups (negative control). Hepatotoxic groups induced with PCM pretreated with silymarin or 500 mg/kg AEMM exhibited significant (P<0.05) decreased in the mean of histological scoring compared to the negative control. On the other hand, only 500 mg/kg AEMM significantly (P<0.05) decreased the mean of scoring for CCl4-induced hepatotoxicity study compared to the negative control group (P <0.05). Liver function test demonstrated significant decreased (P<0.05) of ALT and AST level in PCM-induced hepatotoxicity and also has reducing effect in CCl4 intoxicated group. In conclusion, the aqueous extract of leaves of Melastoma malabatricum exerts potential hepatoprotective property that warrants further investigation.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Mr Muhamad Fazril Awang
Date Deposited:	23 Nov 2022 03:25
Last Modified:	23 Nov 2022 03:25
URI:	http://psaspb.upm.edu.my/id/eprint/378

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