The effects of Curcuma Longa (turmeric) and Levamisole on in-vitro survival rate of strongyles in sheep

Nasai, Norisal (2015) The effects of Curcuma Longa (turmeric) and Levamisole on in-vitro survival rate of strongyles in sheep. [Project Paper] (Submitted)

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Abstract

Gastrointestinal helminth is a global problem in small ruminant production. Most of the parasites were found to develop resistance since chemical anthelminths have been used for a long period of time. The present study evaluated Curcuma longa as a biological nematicide in the treatment of the gastrointestinal helminth especially for strongyles in sheep. The objectives of the study were to compare the efficacy of Curcuma longa and Levamisole on the survival rate of the strongyles in sheep and to determine the most effective concentration of the Curcuma longa extract for larvacidal activity against strongyle third stage larvae (L3) in in-vitro condition. L3 of the strongyles were cultured from the feces of naturally infected sheep and after 7 days, the L3 were harvested from the fecal culture. Methanolic extract of Curcuma longa were prepared into three different concentrations: 50, 100 and 200mg/ml, and the efficacy of the extract on the L3 was tested. Levamisole at concentration 1.5 mg/ml and 3.0 mg/ml were used as the positive control. The Curcuma longa extract showed anthelmintic activity in a dose dependent manner with 78% worms died within 24-hour exposure at the highest dose rate of 200 mg/ml. The mortality rate was compared with Levamisole 1.5mg/ml where 72% worms died after 24-hour exposure. Therefore, there is no significance difference between the effects of using Curcuma longa, 200mg/ml and Levamisole, 1.5mg/ml (p>0.05). There was no mortality of worms in distilled water for a 24-hours observation. Further research could be done to determine the antihelmintic activity of the Curcuma longa extract at higher doses.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	21 Nov 2022 07:36
Last Modified:	21 Nov 2022 07:36
URI:	http://psaspb.upm.edu.my/id/eprint/410

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