Microbiological quality of Cerithidea obtusa and the antibiotic sentibiotic sensitivity of selected bacteria

Hazri, Aina Liyana (2016) Microbiological quality of Cerithidea obtusa and the antibiotic sentibiotic sensitivity of selected bacteria. [Project Paper] (Submitted)

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Abstract

Cerithidea obtusa is a local delicacy also known as siput sedut. Cerithidea obtusa is a filter-feeder therefore may have poor microbiological quality depending on the hygiene and cleanliness of the water environment where they are derived. The microbiological quality of the Cerithidea obtusa available in the local market have not been evaluated. This study was performed to assess the microbiological quality of Cerithidea obtusa, to detect the presence of selected pathogen such as E.coli, Salmonella and Vibrio spp. in Cerithidea obtusa and their antibiotic susceptibility pattern. Fourteen pooled sample of Cerithidea obtusa was purchased from 10 wet markets around the areas of Kuala Lumpur and Selangor and were examined for the microbiological quality, occurrence of E.coli, Salmonella and Vibrio spp. and to determine the antibiotic susceptibility of the isolates. The information on the shellfish origin and source was supplied by the vendor and it was revealed that 14.3% of the shellfish was from Malaysia and 85.7% was from Indonesia. The microbiological analysis revealed that 28.6% of the total sample tested for Standard Plate Count (SPC) of Cerithidea obtusa had mean count that were beyond the limit ( >5,000,000 CFU/g) set by Microbiological Reference Criteria for Food, October 1995 (Food Administration Manual, 1995) for shellfish, while all of the samples tested for Coliform Plate Count (CPC) had mean count of more than 1,000 CFU/g. There was no significant difference in the microbiological quality of Cerithidea obtusa from Malaysia and Indonesia. This indicate that the origin of Cerithidea obtusa may not be associated with the microbiology quality of the produce. E.coli was detected in 21.4% of samples, while 14.2% and 7.21% were positive for Salmonella spp. and Vibrio spp, respectively. All E.coli and Salmonella spp. isolates were resistant to oxytetracycline. More than half (66.7%) of E. coli isolates were resistant to trimethoprim/sulfamethoxazole, florfenicol, azithromycin, ciprofloxacin and ceftriaxone. Half (50%) of the Salmonella spp. isolates were resistant to trimethoprim/sulfamethoxazole, florfenicol, amoxycillin, ciprofloxacin and ceftriaxone. The isolated Vibrio spp. was resistant to erythromycin only. In conclusion, the study suggests that the local Siput sedut have poor microbiological quality and harbour pathogens that exhibited multidrug resistant trait. More studies need to be performed to investigate the source of the contamination such as water quality and Cerithidea obtusa handling .

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	18 Oct 2022 06:57
Last Modified:	08 Mar 2023 08:55
URI:	http://psaspb.upm.edu.my/id/eprint/563

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