Ahmad, Atikah Kazimah (2011) In-vivo anti inflammatory studies of polypeptide-k and essential oil from momordica charantia seeds (bitter gourd). [Project Paper] (Submitted)
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Abstract
Inflammation is a physiological response of a body to stimuli including infections and tissue injury. There have been reports that prolonged use of anti-inflammatory drugs (NSAIDs) will lead to some adverse effect. Momordica charantia is one of the natural sources that claim having anti-inflammatory properties. This study is to evaluate the effects of polypeptide-k compound and essential oils from Momordica charantia seeds on induced inflammation in experimental rats. Anti inflammatory activity of polypeptide-k and essential oils were evaluated by carrageenan-induced paw edema and carrageenan-air pouch model. A total of six groups containing six rats in a group were used throughout the experiment for both models. Group 1 received 5ml/kg distilled water (negative control), group 2 received 10mg/kg indomethacin as positive control meanwhile group 3 and 4 received 10mg/kg and 50mg/kg polypeptide-k compound respectively whereas, group 5 and 6 received 1ml per rat and 2ml per rat of essential oil respectively. All rats in each group were given treatment orally for duration of four days. In the paw edema study, the final oral administration of each treatment was given on the fourth day 30 minutes before 0.1 ml of 1% carrageenan was injected into the hind paw. The paw volume was measured continuously every hour for duration of 5 hours by using plethysmometer. For the air pouch model, the rats were injected subcutaneously with sterile air on their back to form pouches. On day 4, the rats received pretreatment according to their group. 0.5% carrageenan was injected into the pouches to induce inflammation. After 6 hours, the pouches were washed and fluid collected to determine exudates volume, interleukin 10, total and absolute leukocyte counts and nitrite levels. In paw edema study, there is significantly reduction in paw edema volume at 60 minutes (p<0.05) the positive control group, 50mg/kg polypeptide-k compound, 1ml essential oil and also 2ml essential oil as compared to negative control group. The maximum mean paw volume was observed at 60 minutes after carrrageenan injection (3.00±0.13). At 60 minutes after carrageenan injection, the group treated with 50mg/kg polypeptide-k compound shows higher percentage inhibition of edema (63.29%), followed by 10mg/kg polypeptide-k compound (46.24%), 2ml essential oil administration (41.33%), 1ml essential oil administration (36.13%), and lastly positive control group (5.78%). In the air pouch study, there is significant suppression of total and absolute leukocytes count (p<0.05) seen in the treatment groups. Neutrophils and monocytes counts were significantly decreased (p<0.05) in treatment groups except in 10mg/kg polypeptide-k group. The group treated with 1ml essential oil shows higher percentage inhibition of leukocytes count (69.76%) when compared with negative control group followed by 50mg/kg polypeptide-k compound (63.95%), 2ml essential oil (62.81%), 10mg/kg polypeptide-k compound (49.32%) and lastly positive control group (48.89%). There is significant decrease (p<0.05) of nitrite concentration in positive control and 50mg/kg polypeptide-k groups. The concentration of nitrite in negative group is 0.25±0.049µmol/gram which is significantly higher (p<0.05) than other treated group. Meanwhile for the interleukin 10 (IL-10) concentrations in all treated groups were not significantly different from control. The outcome of this study indicates that the polypeptide-k compound and essential oil extracted from Momordica charantia seeds having potential in inhibition of paw edema volume and also have the ability to inhibit acute inflammation by reducing the total leukocytes count and production of nitrite. When compared between polypeptide-k compound and essential oil extraction, it shows that 50mg/kg polypeptide-k compound is more potent that essential oil in inhibiting acute inflammation in rats.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Mr Muhamad Fazril Awang |
| Date Deposited: | 22 Nov 2022 06:14 |
| Last Modified: | 22 Nov 2022 06:14 |
| URI: | http://psaspb.upm.edu.my/id/eprint/605 |
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