Pathogenicity assessment of cellular membrane products (CMPs) and extracellular membrane products (ECPs) of Aeromonas hydrophilia towards African catfish (Clarias gariepinus)

Shariruzi, Humairak (2016) Pathogenicity assessment of cellular membrane products (CMPs) and extracellular membrane products (ECPs) of Aeromonas hydrophilia towards African catfish (Clarias gariepinus). [Project Paper] (Submitted)

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Abstract

Cell membrane products (CMPs) are made of bacterial cell pellet which consisted of most abundant surface structure termed S-layers. While extracellular products (ECPs) are bacterial supernatant made of haemolysin, enterotoxin, cytotoxin and protease. However, there was lack of previous study done on the effect of CMPs and ECPs towards pathogenicity of fish. This study aimed to determine whether CMPs and ECPs of A. hydrophilia stimulate the pathogenicity of African catfish (Clarias gariepinus). Aeromonas hydrophilia was cultured in Tryptic Soy Broth (TSB) and harvested by centrifugation at 1800 xg for 15 minutes to separate the CMPSs and ECPs of bacterial suspension. They were diluted into 10-1, 10-2, 10-3, 10-4 and 10-5 suspension and stored chill prior to use. The CMPs and ECPs were then injected into fingerlings intraperitoneally, including normal saline as a control. The fish sera were collected at 8 dpi from caudal peduncle vein. Presence of antibody in the fish’s sera were detected using Agar Gel Precipitation Test (AGPT). Clinical signs such as lethargy, severe fin rot, corneal opacity, exophthalmia, eye-ball rupture and erosion of epidermis were noticed. CMPs was more virulence than ECPs based on cumulative mortality. Statistically, there was no significant different in cumulative mortality between CMPs and ECPs. There was no Ab-Ag reaction in AGPT test indicated CMPs and ECPs unable to stimulate immunity in fish at 8 dpi. In a conclusion, it was postulated that the CMPs and ECPs were able to induce marked clinical signs and stimulate short period of immunity.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	21 Dec 2022 03:13
Last Modified:	12 Jan 2023 03:28
URI:	http://psaspb.upm.edu.my/id/eprint/672

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