Detection of haemoparasite in semi-commercial and scavenging village chicken

Aman, Nur Izzati (2017) Detection of haemoparasite in semi-commercial and scavenging village chicken. [Project Paper] (Submitted)

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Abstract

Village chicken (Gallus gallus domesticus) is usually associated with free-range system that exposed to the environment and seasonal outbreaks of disease, as well as to bacteria and parasites found in the soil, wild birds, and other animals. Haemoparasites infections are more prevalent and contribute significantly in village chicken. Therefore, the presence of haemoparasite infection in semi commercial village chicken and scavenging village chicken were compared. A total of 24 blood samples were collected in a village located in Simpang Renggam, Kluang, Johor. 12 blood samples were collected from semi-commercial village chicken from Aqil Aqilah Farm and 12 blood samples were collected from village chicken that were found scavenged around within the village. In this study, we used both microscopic and molecular analysis techniques to examine blood samples for diagnosing avian haemoparasites. Giemsa-stained thin blood smears were prepared and screened for the presence of haemoparasites. Antigen detection using conventional reverse transcriptase PCR for both groups of blood samples was done and specific primer was designed targeting mitochondrial cytochrome b genes of the parasites. During microscopic examination of blood smears, it revealed that there was absence of haemoparasites in all samples obtained. RT-PCR analysis results gave negative finding in all blood samples tested. In conclusion, there are no difference in blood parasite infection in semi-commercial village chicken and scavenging village chicken.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	17 Jan 2023 07:52
Last Modified:	17 Jan 2023 07:52
URI:	http://psaspb.upm.edu.my/id/eprint/710

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