Liew, Hui Xian (2017) Comparison of serum and oral fluid antibody responses against porcine reproductive and respiratory syndrome virus (PRRSV) by using ELISA. [Project Paper] (Submitted)
![[img]](http://psaspb.upm.edu.my/style/images/fileicons/text.png) |
Text
lp FPV 2017 31.pdf Download (1MB) |
Abstract
Porcine Reproductive and Respiratory Syndrome (PRRS) is endemic in Malaysia. It is a disease of concern as it can cause great economic loss due to reproductive failure in sows, high pre-weaning mortality in piglets and respiratory disease in growing and finishing pigs. Therefore, it is necessary to have an efficient and effective surveillance method to detect the disease. In this study, antibody responses against PRRS in oral fluid and serum were compared using IDEXX ELISA test kit. This study was conducted in two commercial pig farms. 32 head of six weeks old animals were used as subject from each farm. These 32 animals were divided into four pens. 8 serum samples and 3 pen based oral fluid sample were collected from each pen. The samples were collected again from the same individual and same pen four weeks later. The serum and oral fluid samples collected were tested using IDEXX PRRS X3 Antibody Test Kit and IDEXX PRRS Oral Fluid Antibody Test Kit respectively. Statistical analysis shows that there is a significant, very strong and positive correlation between serum and oral fluid samples for Farm A (r = 0.962, p < 0.01). There is also a significant, very strong and positive correlation between serum and oral fluid samples for Farm B (r = 0.933, p < 0.01). In conclusion, oral fluid is a useful tool for PRRS surveillance in the farm and a good option alternative to replace the traditional serology method.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 17 Jan 2023 08:25 |
| Last Modified: | 17 Jan 2023 08:25 |
| URI: | http://psaspb.upm.edu.my/id/eprint/713 |
Actions (login required)
 |
View Item |