Burhan, Mohammad Arif Izuan (2017) Pathogenicity and immunogenicity of infectious bursal disease virus attenuated in BGM-70 cell lines in specific pathogen free chickens. [Project Paper] (Submitted)
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Abstract
Infectious bursal disease (IBD) is a contagious viral disease characterized by high mortality and immunosuppression that able to cause severe economic losses to the poultry industry. Control of IBD is mainly by practicing good biosecurity and vaccination programme. It was objective of the study to determine the pathogenicity and immunogenicity of very virulent IBD virus (vvIBDV) attenuated in BGM-70 cell lines in the specific pathogen free (SPF) chickens for the development of IBD tissue culture based vaccine against vvIBDV infection. One day old SPF chicks were divided into 5 groups namely the groups A, B, C, D, and E. The groups A and B were inoculated (0.1mL) with UPM190 IBDV isolate (109.7 TCID50 / 1.0 mL) passaged 10 and 15, via subcutaneous route, respectively. Meanwhile, groups C and D were inoculated (0.1mL) with UPM0081 IBD isolate (109.5 TCID50 / 1.0 mL) passaged 10 and 15 via subcutaneous route, respectively. The group E acted as the control group and was not inoculated with the virus. The chicks were provided feed and water ad libitum. Five chicks from each virus inoculated group were sacrificed on 14 and 28 days of age. Meanwhile five chicks from control group were sacrificed on 1, 14 and 28 days of age. Before sacrificing, the body weight was recorded and serum samples were collected to determine the antibody titer using enzyme linked immunosorbent assays (ELISA). On necropsy, gross lesions were observed and weight of bursa of Fabricius was recorded. The study showed that no mortality and clinical signs were observed in all inoculated and control group throughout the experiment. Same goes to the gross lesion of the bursal of Fabricius. The bursal to body weight ratio was significantly reduced (p<0.05) in groups A and D when compared to group E on 14 days of age. However, there was no significance difference (p>0.05) in all groups on 28 days of age. Bursal lesion score throughout the experiment remain normal to mild (lesion scoring of 0 to 1). IBD antibody titer from ELISA test was negative for all groups throughout the experiment. It was concluded that the live attenuated vvIBDV in BGM-70 cell lines was ineffective in induction of lesions in the bursa of Fabricius and IBD antibody titer.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 21 Feb 2023 03:34 |
| Last Modified: | 21 Feb 2023 03:34 |
| URI: | http://psaspb.upm.edu.my/id/eprint/733 |
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