Ishak, Nur Ain Syahira (2017) Investigation of malachite green residues in local and imported Pangasius hypopthalmus sold in Selangor markets. [Project Paper] (Submitted)
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Abstract
Malachite green is an N-methylated diaminotriphenylmethane dye widely used for therapeutic purposes in the aquaculture sector across the world. Malachite green can easily oxidize to leuco-malachite green (LMG) which remains in muscles and internal organs for several weeks. If fish with MG residues are consumed, it may be harmful to humans as it is toxic and carcinogenic. This study was aimed to detect MG residues in local freshwater fish Pangasius hypopthalmus and imported frozen fillet of the same species from Vietnam. A set of local fish and imported fillet were purchased from seven supermarkets in Selangor. The muscle was analysed for MG residues using enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC-MS). The ELISA standard curve did not have a linear calibration with correlation coefficients lower than 0.9999 (0.5355), causing inaccuracy of calculating the MG concentration. Whereas LC-MS standard curve was near 1 (0.999) and showed residues of MG and LMG ranging from 1.19 to 4.09 µg/kg concentration in 7 samples (4 locals and 3 imported). The minimum required performance limit (MPRLs) for MG and LMG in fish set by the European Union is 2 ppb which means the detected levels in the present study were higher in some samples with an indication that MG is being used indiscriminately in aquaculture.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 08 Feb 2023 03:16 |
| Last Modified: | 08 Feb 2023 03:16 |
| URI: | http://psaspb.upm.edu.my/id/eprint/735 |
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