Histological changes in the liver of goats with ketosis

Balan, Shogashan (2017) Histological changes in the liver of goats with ketosis. [Project Paper] (Submitted)

Text lp FPV 2017 83.pdf Download (3MB)

Abstract

The demand for goat milk and meat in Malaysia has been reported to increase due to rapid population growth, urbanisation together with changing consumer preference. However, the dairy production of small ruminants is not fulfilling the demand. One of the major factors that restrict the production is the metabolic disorder known as ketosis. This case-control study is done to identify the histopathological lesions in liver of goats clinically diagnosed with ketosis and to associate the histopathological changes with the clinical features. The study was carried out without knowing the clinical background of the cases. A total of 9 liver samples including control cases were fixed in 10% buffered formalin. Histological procedure was done with sectioning, processing and sectioning at 4µm before staining with haematoxylin and eosin. Histological examination was carried out and severity was graded using the percentage of lesion distributed. The lesions found were fatty liver, congestion and thrombosis. These lesions were graded into mild, moderate and severe and further analysed using Mann-Whitney U test. The fatty liver lesion was statistically significant (p<0.05) compared to congestion and thrombosis, which were not significant (p>0.05). The clinical signs observed included dull, depression, teeth grinding and recumbency while the blood glucose and serum β- hydroxybutyric acid (BHBA) were measured. Frequency data analysis revealed that clinical signs were observed only in ketosis group. Blood glucose level of ketosis group was significantly (p<0.05) lower than control group while BHBA of ketosis group was significantly (p <0.05) higher than control group. Correlation analyse revealed that the BHBA have negative correlation with glucose but positive correlation with histological score, similarly histological scoring showed negative correlation with glucose level. In conclusion, the fatty liver is strongly associated with ketosis, particularly severe ketosis with clinical signs. Significant differences were detected between ketosis group and biochemistry parameters, which were BHBA and glucose. Therefore, there is high likely that a goat exhibiting fatty liver lesion also suffering from severe clinical ketosis with clinical signs.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	01 Mar 2023 03:27
Last Modified:	01 Mar 2023 03:27
URI:	http://psaspb.upm.edu.my/id/eprint/788

Actions (login required)

View Item