Identification and antibiotic sensitivity profile of bacteria from catfish, Clarias spp. obtained from selected markets in Selangor

Rosman, Ilia Iryani (2017) Identification and antibiotic sensitivity profile of bacteria from catfish, Clarias spp. obtained from selected markets in Selangor. [Project Paper] (Submitted)

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Abstract

Clarias sp. also known as catfish is a very popular freshwater fish in Malaysia. Wild catfish are most commonly encountered in muddy or swampy water of high turbidity. For human consumption, catfish species are normally reared in ponds and often get contaminated by a variety of microorganism because of the way they are being reared and the environment. Thus, these fish are highly likely to carry pathogens and can be unsafe for human consumption if the fish is not cooked well. This study was conducted to isolate and identify the types of bacteria found in catfish, Clarias spp. and to determine the antibiotic susceptibility pattern of the common bacteria found in the fish, Clarias spp. Twenty-four Clarias spp. samples were purchased from 8 selected markets around the areas of Selangor for a period of two weeks. The gills and intestines were processed for isolation and identification of bacteria. The antibiotic susceptibility of the obtained isolates was also determined against the commonly used antibiotics in aquaculture. A total of twenty Gram negative (41.67%) and seven Gram positive bacteria (14.58%) were isolated from the samples. The gram negative bacteria isolated were Aeromonas species (37.5%), Vibrio cholarae (31.25%), Escherichia coli (16.66%), Klebsiella pneumonia (12.5%), Vibrio parahemolyticus (10.41%), Proteus vulgaris (10.41%), Pantoea agglomerans (10.41%), Proteus mirabilis (10.41%), Enterobacter aerogenes (6.25%), Photobacterium damsela (6.25%), Edwardsiella tarda (6.25%), Pleisiomonas shigelloides (6.25%), Citrobacter freundii (6.25%), Salmonella species (2.08%), Vibrio alginolyticus (2.08%), Chromobacterium (2.08%), Achromobacter (2.08%), Shewanella putrefaction (2.08%) and Alcaligenes fecalis (2.08%). While, the Gram positive bacteria species isolated were Staphylococcus species (6.25%), Streptococcus viridans species (4.16%), Actinomyces species (4.16%), Staphylococcus hyicus (2.08%), Corynebacterium species (2.08%), Corynebacterium kutcheri (2.08%), and Listeria monocytogenes (2.08%). Multidrug resistant traits were presented for 4 Gram negative bacteria species and 1 Gram positive bacteria species. As a conclusion, this study suggested that the catfishes harbours pathogens that exhibited multidrug resistant trait which may bring risk to the consumers.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	01 Mar 2023 03:50
Last Modified:	01 Mar 2023 03:50
URI:	http://psaspb.upm.edu.my/id/eprint/793

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