Determination of fatty acid profile in Scortum barcoo (Jade perch)

Ilias, Amirul Nazhan (2018) Determination of fatty acid profile in Scortum barcoo (Jade perch). [Project Paper] (Submitted)

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Abstract

Marine fishes are better sources of omega-3 polyunsaturated fatty acids (n-3 PUFAs) especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These omega-3 fatty acids can also be found in freshwater fish species, but the composition may be varied. In freshwater fish, omega-3 fatty acid need to be supplemented in the diet as the fish cannot be produced it. Thus, the aim of this study was to determine the relationship between feed and fatty acid profile in freshwater Scortum barcoo (jade perch). The fatty acid profile of six adult Scortum barcoo (n=6) and feed sample were determined by involving three parts; 1) total lipid extraction, 2) fatty acid methyl esters (FAME) preparation and 3) gas-liquid chromatography. Results showed that only monounsaturated fatty acid such as oleic acid (C18:1n-9) was detected in the feed (0.38%) by the gas chromatography. However, in the fish meat, the total of PUFA was lower (4.24%) than the total SFA (6.96%). Apart from that, the total omega-3 PUFA was 1.70% and the total of omega-6 PUFA was 2.54%. In addition, the ratio of omega-3 and omega-6 was 1:2 in the fish meat. Surprisingly, the DHA and EPA were found in the fish meat but not in the feed at 1.45% and 0.25%, respectively. In conclusion, this study revealed that the freshwater Scortum barcoo is capable to convert from short chain carbon such as oleic acid (C18:1n-9) found in the feed to long-chain carbon DHA (C22:6n-3) found in the fish through the enzymatic process of elongation and desaturation of the fatty acid hydrocarbon chain. This could indicate that the fresh water Scortum barcoo may depends on the feed given which contributes to the fatty acid profile.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	03 Mar 2023 03:21
Last Modified:	03 Mar 2023 03:21
URI:	http://psaspb.upm.edu.my/id/eprint/826

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