Molecular detection of norovirus in porcine, canine and feline in Selangor, Malaysia

Tan, Wei Yang (2020) Molecular detection of norovirus in porcine, canine and feline in Selangor, Malaysia. [Project Paper] (Submitted)

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Abstract

Norovirus (NoVs) belongs to the Caliciviridae family, non-enveloped, positive sense, and single-stranded RNA viruses. Norovirus is the leading cause of nonbacterial gastroenteritis in humans worldwide. Despite the world-wide norovirus detection, there is still no data on norovirus on porcine, canine and feline in Malaysia. Thus, this study aimed to describe norovirus locally in healthy animals which include porcine, canine and feline. In this study, 24 pigs including 12 weaners and 12 sows, 20 dogs and 20 cats faecal samples were selected for this study. The faecal samples of porcine were collected from 2 pig farms in Selangor, while faecal samples for canine and feline were collected from an animal shelter in Selangor. Faecal samples were processed according to standard virology and molecular methods and subjected to RT-PCR assay. There were 4 published primers used consisting of universal primers targeting the RdRp region (1 set) and species-specific primers targeting the capsid region (3 sets). Results revealed that 0% (0/46), 0% (0/20), and 0% (0/20) of the porcine, canine and feline were positive for NoVs antigen, respectively. This finding, however, does not rule out the absence of norovirus in animals, as the virus is prevalent in several countries. Unsuccessful detection of NoVs in faecal samples could be attributed mainly to sampling of healthy animals where according to report NoV shedding in faecal last for only 5 days during acute infection. Thus, it is highly recommended for the samples to be obtained from acutely infected animals with gastroenteritis signalment. Alternatively, serological study could be done by detecting antibodies against NoVs to detect animals that have past exposure to NoVs. In conclusion, NoVs antigen could not be demonstrated in clinically healthy porcine, canine and feline in Selangor.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Oct 2023 08:05
Last Modified:	19 Oct 2023 08:05
URI:	http://psaspb.upm.edu.my/id/eprint/1496

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