Zulkepli, Nur Ainaa Husna (2023) Occurrence of ectoparasitic infections and its histological changes in spotted green pufferfish, dichotomyctere nigroviridis (marion de procé, 1822) in Bagan Lalang, Selangor. [Project Paper] (Submitted)
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Abstract
The spotted green pufferfish, Dichotomyctere nigroviridis was able to live in both freshwater and brackish water, and it can be easily found in the estuaries of Malaysia. The parasitic fauna in the pufferfish may provide further information on the host-parasite interaction that may either have a detrimental effect on it or none at all. The purpose of this study was to detect the presence of ectoparasites harboring the skins, fins, and gills of the Dichotomyctere nigroviridis and the effects on histopathological changes. A total of 16 pufferfish comprising 3 juveniles and 13 adults were collected from the sampling site in Bagan Lalang, Selangor through fishing and collection from the fishermen. The skin scraping, fin scrapping and gills samples were collected for direct wet mount to identify the ectoparasites. The skin and gills samples were taken and preserved in Davidson’s solution histopathological evaluation process. The number of parasites between juveniles and adults was used for statistical analysis using a t-test in GraphPad Prism version 10. The histopathological evaluation was done by dividing the lesions based on 5 different criteria which were lamellar hyperplasia, lamellar fusion, cellular anomalies, and lamellar edema with scores from 0 to 3, and ancillary criteria for the presence and absence of ectoparasites. For histopathological analysis, the same t-test was also used to compare the histopathological score for each criterion between juveniles and adults, between the number of parasites in adults and juveniles with the histopathological score, and also for the skin histopathological score between juveniles and adults. The ectoparasites found in this study was Dactylogyrus spp. mainly found in the gills. This study also revealed that there was no significant difference in parasites between adults and juveniles. However, there was a significant difference between the number of parasites in adults with histopathological changes. Among the different kinds of histopathological scoring, cellular anomalies recorded the highest score with about 90% of the sample having degeneration of cells followed by ancillary criteria with about 80% of fish samples observed. All the findings from the Dichotomyctere nigroviridis can be used as preliminary ectoparasite investigation in pufferfish and for future studies of the species.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 12 Aug 2024 03:23 |
| Last Modified: | 12 Aug 2024 03:23 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2045 |
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