Extraction of antioxidant from propolis of stingless bee

Mazlan, Mohammad Tawfiq (2018) Extraction of antioxidant from propolis of stingless bee. [Project Paper] (Submitted)

Text FK 2018 27.pdf Download (66MB)

Abstract

Propolis has constantly been utilized as useful food for its outstanding health effect. However, limited literature is available regarding the antioxidant activity of the stingless bee products in Malaysia especially for the propolis. This is due to its variability in species and chemical diversity of propolis produced by each stingless bee. In order to improve the efficiency of antioxidant extraction of propolis, an experiment was carried out to optimize the extraction conditions by varying the amplitude, cycle and extraction time of a sonication technique which by several antioxidant assays; DPPH Radical Scavenging Activity Assay (DPPH), Ferric Reducing Antioxidant Power (FRAP) and Total Phenolic Content (TPC) were analysed. The extraction process of bioactive compound from propolis was optimized by using central composite rotatable design (CCRD) of Response Surface Methodology (RSM) to evaluate the effect of factors investigated. DPPH Radical Scavenging Activity, Ferric Reducing Antioxidant Power (FRAP) and Total Phenolic Content (TPC) predicted by model for the propolis extract were 86.79%, 1015.63 mM FeSO4 /g and 13.66 mg GAE/g respectively. The suggested condition for extraction of bioactive compounds was obtained in higher levels: amplitude 52.40%, cycle 53% and extraction time 31. 37min. The validation experiments performed gave radical scavenging activity of DPPH values 78.52% which is 10.53% deviation, FRAP values of 722.76 mM FeSO4 /g which is 40.52% deviation and TPC values of 12.93 mg GAE/g which is 5.65% deviation respectively.

Item Type:	Project Paper
Subjects:	T Technology > TP Chemical technology
Faculty:	Faculty of Engineering
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	14 Nov 2025 08:19
Last Modified:	18 Nov 2025 02:57
URI:	http://psaspb.upm.edu.my/id/eprint/2593

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