Isolation and screening of potential antagonistic bacteria against Colletotrichum gloeosporioides, the causal agent of anthracnose disease in papaya

Radi, Nur Aimi (2009) Isolation and screening of potential antagonistic bacteria against Colletotrichum gloeosporioides, the causal agent of anthracnose disease in papaya. [Project Paper] (Submitted)

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Abstract

The objective of this project is to screen the potential antagonistic bacteria to be used as a biological control for Collelotrichum gloeosporioides, the causal pathogen of anthracnose disease in papaya. Fifteen antagonistic bacteria were isolated from infected papaya fruit's surface and screened by dual and concomitant test. There is significant different of inhibitory effect at p<0.05 for dual culture assay test. Two isolates of bacteria (UPMKB4 and UPMKB6) had potential antagonist action against C. gloeosporioides. Mycelia growth test also showed a significant different for percentage of mycelia growth at p<0.05. Bacteria UPMKB4 and UPMKB6 inhibited the fungal growth by an average of 53.57 and 51.90%, respectively during in-vitro screening on PDA medium. Malformation of hyphae occurred in the presence of both bacteria. Hyphae were shrunk when treated with UPMKB6 and swelled when treated with UPMKB4. C. gloeosporioides treated with bacteria also showed the significant different at p<0.05 in reduction of the spore germination. Treatment with UPMKB3 showed the lowest percentage of germinated spore (45.43%). There is significant different at p<0.05 obtained in the fruits detached assay treatment. Antagonistic bacteria could reduce the growth and effect of C. g/oeosporioides. UPMKB4 and UPMKB6 sprayed onto infected papaya showed the percentage of anthracnose disease severity of 53%, which categorized in the moderate condition, compared to 81 % in control treatment. Therefore, in-vitro activities of UPMK.84 and UPMKB6 against C. gloeosporioides in this project suggested that these antagonistic bacteria can be used as an effective biological control agent.

Item Type:	Project Paper
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	08 Dec 2025 03:44
Last Modified:	08 Dec 2025 03:44
URI:	http://psaspb.upm.edu.my/id/eprint/2617

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