Genetic diversity study of selected Sarawak rice cultivars using microsatellite markers

NEOH, PHENE PEI NEE (2009) Genetic diversity study of selected Sarawak rice cultivars using microsatellite markers. [Project Paper] (Submitted)

Text lpFSPM2009 97 1900127871 ADA PW.pdf Download (8MB)

Abstract

Sixteen selected Sarawak rice cultivars were evaluated for genetic diversity using twelve simple sequence repeat (SSR) markers. Forty-nine microsatellite markers were screened and twelve polymorphic SSR markers were selected for further analysis using polyacrylamide gel electrophoresis. A total of 34 alleles were detected by using 12 polymorphic markers. Polymorphism information content (PIC) values of the markers ranged 0.375 (RM489) to 0.703 (RM1) with an average value of 0.562. The dendrogram (r=0.78) based on SSR polymorphism indicated great variation among the rice cultivars, with similarity coefficients ranging from 0.262 to 1.000. Genetic diversity, determined by cluster analysis, indicated two clusters at 26.21% level of similarity with additional sub-groups within each cluster. This study revealed that SSR markers are a useful tool for cultivar differentiation according to their genotypes. The information on genetic diversity of rice genotypes is essential for proper identification and selection of appropriate cultivars for rice improvement.

Item Type:	Project Paper
Subjects:	S Agriculture > S Agriculture (General)
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Mohd Faizal Jabat
Date Deposited:	11 May 2026 02:19
Last Modified:	11 May 2026 02:19
URI:	http://psaspb.upm.edu.my/id/eprint/2767

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