Fermentation condition by Yarrowia lipolytica for erythritol production

Roshara'madan, Ezyan Nazeefa (2022) Fermentation condition by Yarrowia lipolytica for erythritol production. [Project Paper] (Submitted)

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Abstract

Erythritol is a type of sugar alcohol that can be used as a sugar replacement or sweetener. It is a member of the group known as sugar alcohols. A meal consisting of erythritol has close to close to twice as much energy as one consisting of sucrose. Erythritol is an excellent sugar replacement for diabetics since it has either no effect or very little effect on the release of insulin. The use of glucose as a substrate in the synthesis of erythritol is the most common method. With the assistance of yeast Yarrowia /ipolytica, an erythritol production is being carried out in this research with the use of glucose as the substrate. The fermentation experiment was conducted by using the bioreactor with parameter profiles of 250 rpm for agitation speed, temperature at 30°C, 1.5 L/min of airflow, pH­controlled at 5.5 and 0.2% antifoam. At the end of the SO-hour fermentation procedure, the maximum amount of erythritol that was generated was reported to be 98.45 g/L. However, at 54-hour of fermentation, the amount of erythritol that was created began to diminish as a result of the depletion of glucose. The Monad method was utilised in order to arrive at the values for the saturation constant and the maximal specific growth rate. The highest possible specific growth rate for Yarrowia lipolytica was calculated to be 0.059 hour-1.

Item Type:	Project Paper
Subjects:	Q Science > Q Science (General)
Faculty:	Faculty of Engineering
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	13 May 2026 09:32
Last Modified:	13 May 2026 09:32
URI:	http://psaspb.upm.edu.my/id/eprint/2810

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