Antioxidant properties of fresh and frozen peel of lemon, key lime and musk lime as a potent source of antioxidant

Noor Azman, Nur Fatin Inazlina (2018) Antioxidant properties of fresh and frozen peel of lemon, key lime and musk lime as a potent source of antioxidant. [Project Paper] (Submitted)

Text lp FPSK3 2018 9 REPAIR.pdf Download (45MB)

Abstract

Citrus belong to family Rutaceae appear as promising sources of beneficial co1nponents as it contains high phenolic and flavonoid compound. However, citrus peel often being discarded as waste which contain high polyphenol and believed able to protect from chronic diseases. The freezing process may offer better antioxidant yield, polyphenol content and activity based on previous literature. This study aimed to determine antioxidant content and activity of fresh and frozen selected citrus peels. Frozen and fresh peels of lemon, key lime and musk lime were subjected to FolinCiocalteu reagent for phenolic content (TPC) determination and aluminium chloride calorimetric assay for total flavonoid content (TFC) determination. The antioxidant activity determined by DPPH free radical scavenging activity assay and ferric ion reducing antioxidant power assay (FRAP). The TPC of frozen samples were significantly higher than fresh samples (p<0.05) and ranged frotn 72.01 ±0.67 mg GAE/g to 136.48±0.58 mg GAE/g. Similarly, TFC were also significantly different (p<0.05) ranging from 50.51± 1.36 mg QE/g to 178.32±2.13 mg QE/g. The ECso values obtained from DPPH free radical scavenging assay ranged from 0.823±0. l mg/ml to 3.16±0.92 mg/ml with frozen citrus peels having a lower value indicating its higher antioxidant activity as compared to the fresh citrus peels. Furthennore, frozen citrus peels exhibited higher value in FRAP assay compared to fresh citrus peels sample that range from 0.38±0.003 mM Fe2+/g to 0.53±0.009 1nM Fe2+/g with significant different between the fresh and frozen citrus peel samples (p<0.05). For Pearson correlation, there is no correlation between DPPH with TPC and TFC. However, there is positive correlation exist between FRAP with TPC and TFC (r=0.783, r=0.681, respectively). Overall, the present study showed frozen citrus peels possess higher antioxidant activity and content compared to fresh citrus peels. Suggesting frozen citrus peel as more potent source of natural antioxidant.

Item Type:	Project Paper
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	22 Jul 2026 03:26
Last Modified:	22 Jul 2026 04:07
URI:	http://psaspb.upm.edu.my/id/eprint/2876

Actions (login required)

View Item