Abdul Rashid, Nor Afifah (2021) Cytotoxic Effects of Novel Pyrazole Derivatives (BK-35 & HEXUR- 2) on HCT116 Cells Human Colon Cancer Cells. [Project Paper] (Submitted)
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Abstract
Colon cancer is the third most common cancer worldwide and the second leading cause of cancer among male and female. An uncontrolled abnormal growth from primary tumor and dissemination of colon cancer cells to the distant organs are known to contribute to the high mortality rate in colon cancer patients. Current cancer treatments including surgery, chemotherapy and radiotherapy are used for colon cancer. However, there are less effective which sometimes causes patients to induce metastasis, cancer recurrence and drug resistance. Hence, the development of novel therapeutic compound to target cancer cells without damaging the normal tissue is being extensively studied as to discover a new anti-cancer drug. Novel pyrazole derivatives are an active heterocyclic compound with five-membered ring. It have been shown to exhibit anti-cancer properties which a few studies reported on the effects of pyrazole derivative that able to inhibit cell proliferation and migration. Objective: Therefore, the purpose of this study is to determine the cytotoxic properties of novel pyrazole derivatives (BK-35 & HEXUR-2) on HCT116 human colon cancer cells. Methodology: HCT116 cell were cultured in McCoy’s 5A at humidified condition. MTT Assay is used to determine the cell viability and cytotoxic properties of different concentration of BK-35 and HEXUR-2 (1.5625μM, 3.125μM, 6.25μM, 12.5μM, 25μM, 50μM, 100μM, and 200μM) on HCT116 cells at different incubation time points (24, 48 and 72 hours). 5-fluorouracil (5-FU) was used as positive control in this study which cell treated with 10μM (24 and 48 hours) and 7μM of 5-FU (72 hours). The morphological changes of the colon cell line were observed for each concentrations of treatment. Results: From the MTT data obtained, the IC50 values of HCT116 cells treated with BK-35 are 96uM and 70uM at 48 hours and 72 hours of incubation time, respectively. In contrast, there is no IC50 values was obtained at 24 hours incubation time in cells treated with different concentration of BK-35. For HEXUR-2, it showed the cytotoxic effects with the IC50 values are 50uM, 11uM and 8uM at 24, 48 and 72 hours incubation time. In HCT116 cells treated with BK-35 and HEXUR-2, the morphological changes of apoptosis including cell shrinkage, nuclear fragmentation, and membrane blebbing were observed. Discussion: In this study, BK-35 and HEXUR-2 showed a cytotoxic properties towards colon cancer cell line. Morphology study was suggested that the cytotoxic properties of both compounds on colon cancer cell line was mediated by apoptosis mechanism. Conclusion: BK-35 and HEXUR-2 as the novel pyrazole derivatives shown to exhibit cytotoxic properties thus could be a potential candidate for a new anti-cancer agent discovery.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 07:08 |
| Last Modified: | 22 Aug 2023 07:08 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1001 |
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