Subacute Toxicity Study of Purple Sweet Potato (Ipomoea batatas) Leaves Ethanolic Extract (PSPLEE) on Kidney of Sprague Dawley Rats

Saharuddin, Anis Natasha (2021) Subacute Toxicity Study of Purple Sweet Potato (Ipomoea batatas) Leaves Ethanolic Extract (PSPLEE) on Kidney of Sprague Dawley Rats. [Project Paper] (Submitted)

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Abstract

Plants have been shown to possess various benefits, including as a source of medicine. The usage of plants as medicine or herbal remedies has been known for a long time, and it is also profoundly known to be the source of bioactive compounds for modern drugs. Sweet potato (Ipomoea batatas) is a Convolvulaceae family member that has the potential to be used as a therapeutic agent due to its health advantages, as determined by several studies. Some of the potential health benefits include antidiabetic, antioxidant, anti-inflammatory and many more. Despite having numerous findings on the health potential of sweet potato, published reports of its toxic effects are still limited. Objectives: This study aims to investigate the subacute toxic effect of purple sweet potato leaves ethanolic extract (PSPLEE) on the kidney of Sprague Dawley rats as per OECD Guidelines 407 for subacute protocols. Methodology: Collection and extraction of purple sweet potato leaves were performed before the test. The subacute toxicity test was conducted by dividing 30 Sprague Dawley rats into 5 groups (n=6), where one group acted as the control group, and the remaining 4 groups were the treatment groups. Each of the treatment groups received a different dosage of PSPLEE (50, 250, 500, and 1000 mg/kg bw) orally for 28 days. At the end of the treatment, the animals were sacrificed and both blood and renal tissue samples were subjected to renal function tests and histopathological examinations. Results: No signs of toxicity and no deaths were observed in all PSPLEE-treated rats. The body weight and the relative weight of the kidney revealed no significant differences. No significant differences were also observed in all of the parameters in the renal function test, and no microscopic changes were determined in histopathological examination of the kidney tissue samples. Discussion: Absence of significant changes in the body weight and relative of the kidney might indicate that PSPLEE is non-toxic. No significant changes in the parameters for the renal function test may suggest that no harmful effects were triggered by the various dosages (50, 250, 500 and 1000 mg/kg BW) of PSPLEE on the kidney of the treated rats as compared with their respective control group. This finding is supported by the absence of microscopic changes in the histopathology of the kidney. Conclusion: The findings from this study indicate that repeated oral administration of purple sweet potato leaves ethanolic extract (PSPLEE) did not develop any significant toxic effects on the kidney of treated Sprague Dawley rats. However, further toxicity assessment such as chronic, or genotoxic studies using repeated doses of PSPLEE should be Conducted to confirm its safety on prolonged use.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:51
Last Modified:	22 Aug 2023 07:51
URI:	http://psaspb.upm.edu.my/id/eprint/1011

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